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黄芪、枸杞、金银花等中草药复方制剂对肉鸡免疫器官发育及免疫功能的影响
复方中草药 肉鸡 免疫器官 免疫功能
2017/7/12
为了研究由黄芪、枸杞、金银花等药材组成的复方中草药制剂对肉鸡免疫器官发育及免疫功能的影响，将1日龄健康AA肉鸡1 000羽随机分为对照组和试验Ⅰ、Ⅱ、Ⅲ组，每组5个重复，每个重复50羽，对照组饲喂基础日粮，试验Ⅰ、Ⅱ和Ⅲ组分别在基础日粮中添加0.5%、1.0%和1.5%的复方中草药制剂，试验期42 d。结果显示，在21日龄时，试验Ⅱ组肉鸡法氏囊指数、血清IgG和IgM含量以及NDV抗体和IL-4水...
郁金散对大肠湿热证模型大鼠血清及肠道组织胃肠激素的影响
大肠湿热证 郁金散 大鼠 胃肠激素
2017/7/12
探讨郁金散对大肠湿热证模型大鼠血清和肠道组织胃肠激素的影响。Wistar大鼠60只，体重180~220 g，随机分为正常对照组、大肠湿热证模型组、自愈组、郁金散高、中、低剂量治疗组，每组10只。通过自由饮用蜂蜜水；前10 d禁食与饲喂充足饲料并灌胃猪油隔日交替进行；之后5 d每天灌白酒，并置于高温高湿环境（8 h·d-1）；然后腹腔注射大肠杆菌，24 h后再注射一次建立大肠湿热证大鼠模型。造模成功...
本文根据田间发现的黏虫大发生迁飞种群的多数个体均发生了不同程度黑化的现象,结合近年来迁飞昆虫的密度依赖性防御能力、体液免疫与细胞免疫应答机制、防御的神经内分泌调控机制以及生态免疫等方面的研究进展,提出黏虫防御反应及其与迁飞致灾关系的思考,对于揭示迁飞性黏虫成灾新机制以及指导其生物防治均具有重要意义。
Influence of polymer-coated slow-release urea on total tract apparent digestibility, ruminal fermentation and performance of Nellore steers
Slow-release Urea Ammonia Average Daily Gain Non-protein Nitrogen
2017/3/7
Two experiments were performed to evaluate the effects of coated slow-release urea on nutrient digestion, ruminal fermentation, nitrogen utilization, blood glucose and urea concentration (Exp 1), and ...
Dietary chitosan affects metabolism of arachidonic acid in weaned piglets
cytosolic-phospholipase A2 prostaglandin E2 leukotriene B4 5-lipoxygenase cyclooxygenase-2 mRNA expression
2017/2/21
The effects of chitosan on immune function via arachidonic acid (AA) pathway in weaned piglets were investigated. A total of 180 piglets (Duroc × Yorkshire × Landrace) were randomly assigned to 5 diet...
利用蛋白质组学的方法对猪繁殖与呼吸综合征病毒（porcine reproductive and respiratory syndrome virus，PRRSV）感染猪肺泡巨噬细胞（pulmonary macrophage，PAM）后的蛋白质动态变化进行探讨，为阐明病毒感染后引起的复杂免疫学反应及为该病的防控提供理论依据。通过Label-free LC-MS/MS方法定量鉴定PRRSV感染PAMs...
为了验证硒化党参多糖（selenizing Codonopsis pilosula polysaccharide，sCPP）和大蒜多糖（garlic polysaccharide，GP）的协同增强免疫作用及其复方硒化党参多糖-大蒜多糖（sCPP-GP）的功效，为研制新型免疫增强剂提供理论依据，比较了这3种多糖的增强免疫活性。体外试验，用MTT法和RT-PCR法测定了3种多糖对鸡外周血淋巴细胞的增殖...
旨在寻找有效方法，能够从PRRSV感染的MARC-145细胞中分离细胞产生的外泌体，并为探索外泌体在PRRSV感染细胞时的作用奠定基础。使用试剂盒提取MARC-145细胞产生的外泌体，利用电镜负染色法和Western blot技术对MARC-145细胞产生的外泌体进行鉴定；采用碘克沙醇速率区带离心分离MARC-145细胞产生的外泌体，并用Western blot技术检测外分泌体的分布区间。用PRR...
Effects of γ-aminobutyric acid on the thymus tissue structure,antioxidant activity,cell apoptosis,and cytokine levels in chicks under heat stress
heat shock GABA chick thymus immune function development
2016/12/20
This study aims to investigate the effect of dietary γ-aminobutyric acid (GABA) on the development of thymus tissue structure and function in chicks under heat stress. One-day-old male Wenchang chicks...
基因Ⅰ型猪乙型脑炎灭活疫苗免疫原性评价
乙型脑炎病毒,基因Ⅰ型,基因Ⅲ型,灭活苗,免疫原性
2017/5/25
为了评估猪基因Ⅰ型乙型脑炎病毒YA1株、CZ株灭活疫苗的免疫原性，通过将YA1株、CZ株乙脑病毒分别在乳鼠脑内连续传代，获得了高病毒含量的鼠脑病毒液。将佐剂ISA206分别与灭活的YA1株、CZ株乙脑病毒液混合，制备成鼠脑灭活苗。将YA1株、CZ株鼠脑灭活苗以及HW1株商品化鼠脑灭活苗分别免疫接种小鼠、豚鼠、仔猪，采用间接ELISA试验、血凝抑制试验、病毒中和试验以及攻毒保护试验分别检测IgG抗体...
Immunohistochemical analysis of metastasising hepatocellular carcinomas in dogs
cell markers expression CEA CK7 CK20 CD4 CD8 vimentin AFP immunohistochemistry pleomorphic cells liver
2016/11/11
In this study, the immunohistochemical features of primary hepatocellular carcinomas and their metastases in visceral organs, including the lungs, spleen and kidneys were examined using antibodies aga...
Changes in lymphocyte function and subset counts in cats with spontaneous chronic kidney disease
uraemia immunosuppression lymphopenia mitogens flow cytometry
2016/11/11
Chronic kidney disease alters the immune response in humans and dogs and it is thought to be one of the causes of immunosuppression in cats. Haematological, biochemical, and immunological examinations...
Decrease in C-reactive protein levels in rabbits after vaccination with a live attenuated myxoma virus vaccine
abbit Oryctolagus cuniculus myxomatosis ELISA
2016/11/11
The aim of this study was to evaluate the acute phase reaction and immune response of rabbits triggered by vaccination with a live attenuated myxoma virus (MXT) vaccine. Thirteen adult and 11 juvenile...
Effect of silver nanoparticles on the immune,redox,and lipid status of chicken blood
chickens nanosilver immunology antioxidant
2016/11/11
The aim of the study was to analyze how per os application of hydrocolloids of silver nanoparticles (22 nm) and lipid-coated silver nanoparticles (5 nm) affect the immune, redox, and lipid status of t...