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Regional distribution of 5alpha-reductase type 1 and type 2 mRNA along the nonhuman primate (Macaca fascicularis) epididymis
Cynomolgus monkey RT-PCR gene expression
2009/2/17
The 5alpha-reduced metabolites of testosterone, including dihydrotestosterone, are considered the primary regulators of epididymal function. Two genes encode two 5alpha-reductase isozymes. We exam...
Isolation of highly purified type A spermatogonia from prepubertal rat testis
Type A spermatogonia cell separation c-kit cell culture
2009/2/17
We have developed a new method that allows isolation of highly purified type A spermatogonia from prepubertal rats. The procedure is based on the maximal release of spermatogonia from the seminifero...
Immunomagnetic isolation and long-term culture of mouse type A spermatogonia
Germ cells c-kit Ep-CAM magnetic beads Sertoli
2008/12/29
In the mammalian testis, type A spermatogonia proliferate and differentiate into sperm under the tight control of both endocrine and paracrine factors. In order to study the complex process of sperm...
Immortalized testis cell lines from estrogen receptor（ER）alpha knock-out and wild-type mice expressing functional ERalpha or ERbeta
Immortalization HPV E6/E7 in vitro models
2008/12/29
The surprising findings that male mice lacking the estrogen receptor (ER) alpha (alphaERKO) have atrophic testis and are infertile proved that ERalpha is involved in normal testicular function. To o...
Stage and region-specific localization of lipocalin-type prostaglandin D synthase in the adult murine testis and epididymis
lipocalin-type prostaglandin D synthase adult murine testis epididymis
2008/12/25
Lipocalin-type prostaglandin D synthase in semen has been associated with male fertility, although this relationship is not well defined. To gain insight into potential mechanisms, the objective of ...
Initial predominance of the oxidative activity of type I 11beta-hydroxysteroid dehydrogenase in primary rat Leydig cells and transfected cell lines
Initial predominance oxidative activity 11beta-hydroxysteroid dehydrogenase Leydig cells transfected cell
2008/12/25
Glucocorticoids suppress testosterone production in Leydig cells. The level of glucocorticoid action is set within the Leydig cell by the number of glucocorticoid receptors and by the activity of ...
Calcium-Modulated Rod Outer Segment Membrane Guanylate Cyclase Type 1 Transduction Machinery in the Testes
ROS-GC1 GCAP1 S100B signal transduction
2008/12/24
The importance of the second messengers, Ca2+ and cyclic GMP, for the process of fertilization is well established; the mechanisms for their intracellular regulations in the testes are, however, poo...
Restoration of Erectile Capacity in Normotensive Aged Rats by Modulation of Angiotensin Receptor Type 1
Erectile dysfunction Sprague-Dawley rat losartan
2008/12/23
We investigated the effects of systemic modulation of angiotensin 2 on the erectile dysfunction of aged rats. Young and aged (18 months old) male Sprague-Dawley rats were either treated with daily a...
Limited Processing of Pro-Matrix Metalloprotease-2（Gelatinase A）Overexpressed by Transfection in PC-3 Human Prostate Tumor Cells: Association With Restricted Cell Surface Localization of Membrane-Type Matrix Metalloproteinase-1
Tissue inhibitor of metalloproteinase-2 prostate cancer Concanavalin A reverse transcriptase-polymerase chain reaction
2008/12/22
The expression and activation of matrix metalloproteinases (MMPs) by tumor cells is correlated with progression to invasive and metastatic status. The purpose of this study was to examine the role of ...
Implication of Calmodulin-Dependent Phosphodiesterase Type 1 During Bovine Sperm Capacitation
Vinpocetine rolipram cyclic AMP in vitro fertilization motility
2008/12/18
Phosphodiesterases (PDEs) are enzymes that degrade cyclic nucleotides. The calcium-calmodulin dependent PDE type 1 (PDE 1) and the cyclic adenosine monophosphate (cAMP)-specific PDE type 4 (PDE 4) h...
Development of a Cryopreservation Protocol for Type A Spermatogonia
Freezing spermatogonial stem cells transplantation
2008/12/5
The aim of this study was to develop a cryopreservation protocol for type A spermatogonia. Testes from 5- to 7-month-old calves were collected, and type A spermatogonia were isolated using two-step ...