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Transplantation of newborn rat testis under the kidney capsule of adult host as a model to study the structure and function of Leydig cells
newborn rat testis kidney capsule adult host Leydig cells
2009/2/23
Newborn rat testis was transplanted under the kidney capsule of adult castrated and uncastrated male rats to develop and characterize a model system for studies on Leydig cell development. Two weeks...
The sperm chromatin structure assay. Relationship with alternate tests of semen quality and heterospermic performance of bulls
sperm chromatin structure assay alternate tests semen quality heterospermic performance
2009/2/23
Data obtained by the sperm chromatin structure assay (SCSA) on spermatozoa from nine bulls were correlated with fertility, measured by heterospermic performance (-0.94, P less than 0.01) and by al...
An experimental study of the effect of ischemia on testicular structure in the rat. Efficacy of intravenous inosine
ischemia inosine microsurgery testis autotranspiantation
2009/2/20
The damages caused by experimental testicular ischemia lasting 60 minutes have been examined in the rat. The histologic alterations of the germinal epithelium and of the tunica propria are studied...
Ethnic differences in testicular structure and spermatogenic potential may predispose testes of Asian men to a heightened sensitivity to steroidal contraceptives
Spermatogenesis ethnicity
2009/2/17
Spermatogenesis in Asian men appears to be more susceptible to suppression by steroidal contraceptives administered in clinical trials than spermatogenesis in Caucasian men. The objective of this ...
Mouse testicular sulfated glycoprotein-1:sequence analysis of the common backbone structure of prosaposins
Mouse testicular sulfated glycoprotein-1 common backbone structure prosaposins
2009/2/17
We have generated a cDNA encoding the mouse sulfated glycoprotein-1 (SGP-1) by polymerase chain reaction amplification of a mouse testicular Uni-Zap XR cDNA library with two synthetic oligonucleotid...
Effects of heat stress on mouse testicular cells and sperm chromatin structure
Testicular heat stress SCSA
2009/2/17
Scrotal regions of mice were exposed to a 38.0, 40.0, or 42.0 degrees C (+/-0.1) H2O bath for 60 minutes to determine the effects of elevated temperatures on testicular cells and sperm chromatin str...
Early effects of vasectomy on testicular structure and on germ cell and macrophage apoptosis in the hamster
Male contraception vasectomy germ-cell apoptosis macrophage apoptosis testosterone testis
2009/2/17
This study provides quantitative information on the early (up to 3 months) effects of vasectomy on apoptosis in the hamster testis. Groups of five adult male golden hamsters were either bilaterall...
Apical and narrow cells are distinct cell types differing in their structure, distribution, and functions in the adult rat epididymis
Apical and narrow epithelial cells initial segment intermediate zone epididymis rat
2009/2/16
Apical and narrow cells of the initial segment and intermediate zone of the adult rat epididymis were glutaraldehyde fixed and Epon embedded for routine light (LM) and electron (EM) microscopic an...
Structure of the rRNA genes in the hamster sperm nucleus
Nuclear matrix nucleolus DNA structure DNA loop
2009/2/13
We have examined the structure of the major ribosomal RNA (rRNA) genes in the hamster sperm nucleus, using fluorescent in situ hybridization (FISH). The rRNA genes are present as tandemly repeated...
Studies on sperm chromatin structure alterations and cytogenetic damage of mouse sperm following in vitro incubation. Studies on in vitro-incubated mouse sperm
sperm chromatin structure alterations cytogenetic damage of mouse sperm
2009/2/12
Mouse epididymal sperm incubated in Tyrode's T6 fertilization media were analyzed over time for chromosome damage by two methods. First, cytogenetic analysis was done on paternal pronuclei metapha...
Structure and control of a cell-cell adhesion complex associated with spermiation in rat seminiferous epithelium
Cadherins cell adhesions control integrins spermiation
2008/12/29
Spermiation, the release of late spermatids from the Sertoli cell, is disrupted by a number of toxicants. Control of the spermiation process, and the proteins that interact to adhere mature spermati...
Chromatin structure in globozoospermia:a case report
Chromatin structure globozoospermia case report
2008/12/26
Sperm nuclear abnormalities in patients with globozoospermia have not been well characterized and may lead to the high rates of fertilization failure and embryo loss reported in patients with this for...
Characteristics of human sperm chromatin structure following an episode of influenza and high fever:a case study
Characteristics human sperm chromatin structure episode influenza high fever
2008/12/25
Semen samples from a fertile patient presenting with influenza and a 1-day fever of 39.9 degrees C were obtained and analyzed at 18-66 days postfever (dpf) for sperm nuclear proteins, DNA stainabi...
Variability and Laboratory Factors Affecting the Sperm Chromatin Structure Assay in Human Semen
Flow cytometry DNA integrity spermatozoa acridine orange
2008/12/23
During the past decade, the sperm chromatin structure assay (SCSA) has become an important tool for assessing semen quality in the human andrology laboratory. The SCSA uses the metachromatic propert...
Cryopreservation of Ram Semen Facilitates Sperm DNA Damage: Relationship Between Sperm Andrological Parameters and the Sperm Chromatin Structure Assay
Artificial insemination semen quality motility chlortetracycline viability SCSA
2008/12/22
We hypothesized that cryopreservation and incubation in conditions that mimic the female genital tract following insemination increases the susceptibility of ram sperm DNA to denaturation. Ram spe...