搜索结果: 1-7 共查到“医学生物化学 ELISA”相关记录7条 . 查询时间(0.015 秒)
化学发光法与ELISA法对HBsAg和HBsAb同时阳性标本检测比较
肝炎病毒,乙型 化学发光测定法
2012/10/18
比较化学发光法(CLIA)和酶联免疫吸附试验(ELISA)在检测HBsAg和HBsAb同时阳性标本的结果,评价其是否具有一致性。方法 用4种不同浓度HBsAg标准品和4种不同浓度HBsAb标准品两两配合成16份样本,用CLIA、ELISA法分别检测,并于24 h后再次检测;同时用4种不同浓度HBsAg血清标本和4种不同浓度HBsAb血清标本两两配合成16份样本进行同样检测。结果 两种方法HBsAg...
Purification and evaluation of somatic, excretory-secretory and Cysteine proteinase antigens of Fasciola Hepatica using IgG-ELISA in diagnosing Fascioliasis
Purification evaluation of somatic excretory-secretory Cysteine proteinase antigens of Fasciola Hepatica IgG-ELISA diagnosing Fascioliasis
2010/1/5
Fasciolosis, or liver fluke disease, caused by parasites of the genus Fasciola is emerging as an important disease in man and animals, in the world and Iran, particularly in nortern parts. The economi...
关于ELISA若干问题的分析
ELISA 空白对照 阴性对照 cut-off值
2009/11/19
简要分析了在ELISA检测技术应用中遇到的空白对照的设立方法、阴性对照的标准、酶标反应板的差异及孵育以及酶标仪的调零与比色、cut-off值的确定、阳性与阴性灰带率、ELISA检测试剂的标准化等一些新问题,重点探讨了ELISA检测技术中传统计算cut-off值的方式所存在的缺陷与形成原因,并提出了适宜的计算cut-off值的方式。
间接ELISA法对H pylori免疫牛初乳中抗体的检测
间接ELISA 幽门螺杆菌 初乳
2009/8/17
目的: 建立间接ELISA法测定牛初乳中H pylori抗体的水平, 并观察特异性抗体的产生规律. 方法: 本试验以H pylori全菌抗原免疫妊娠母牛, 每头牛每次肌肉注射4×10(9)CFU全菌抗原, 收集分娩后7 d内的初乳. 以间接ELISA法检测其中H pylori抗体水平. 结果: H pylori能有效地引起免疫应答, 成年牛初乳中的抗体效价在分娩当天达到最大值5.84, 高于青年牛...
Measurement of Urine Albumin Levels With A Monoclonal Antibody Based in-House Elisa
Human albumin Monoclonal antibody Polyclonal antibody ELISA
2009/6/25
Microalbuminuria is considered to be an early indicator of diabetic nephropathy. We have previously reported the establishment of A-HAlb/98 hybridoma secreting monoclonal antibody that specifically re...
Development of ELISA Systems for Measurement of Human Tumor Necrosis Factor-Alpha (TNF-a)
TNF-a,ELISA
2009/6/22
The aim of this study was to develop a human tumor necrosis factor alpha (hTNF-a) ELISA system because specific and sensitive measurement of low levels of circulating TNF-a is very important for enlig...
Development of a Highly Sensitive ELISA for Quantification of Hepatitis B Virus (HBV) Surface Antigen (HBsAg)
HBsAg monoclonal antibody ELISA
2009/6/17
Background: Hepatitis B Virus (HBV) infection is of global importance and many studies are aimed to develop more sensitive diagnostic tools and improve therapeutic options. HBV infection is diagnosed ...