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Serotonin, GnRH-A, and dopamine interaction in the control of in vivo luteinizing hormone release in Prussian carp (Carassius gibelio Bloch) at the time of gonad recrudescence
gonadotropin-releasing hormone analoque LH
2015/7/7
The effects of serotonin (5-HT), GnRH analogue (D-Ala6
LHRH, GnRH-A) and dopamine
antagonist – pimozide (PIM), on luteinizing hormone (LH) release in mature Prussian carp female (Carassius
gibel...
Interaction between oviposition time, age, and environmental temperature and egg quality traits in laying hens and broiler breeders
hen production type age temperature oviposition
2015/6/23
An experiment with laying hens and broiler breeders of two ages (young 22 vs. 36 weeks, old
83 vs. 64 weeks) and at temperatures of 20 and 28°C was carried out to evaluate the interactions between
...
Evaluation of reference genes for gene expression studies in pig muscle tissue by real-time PCR
gene stability reference genes quantitative PCR Sus scrofa age categories of pigs muscle tissue
2015/6/9
The selection of reference genes is essential for gene expression studies when using a real-time quantitative polymerase chain reaction (PCR). Reference gene selection should be performed for each exp...
Single fixed-time laparoscopic intrauterine insemination as a tool to obtain low-diversity porcine embryos
laparoscopic insemination embryo diversity pig
2015/4/16
Double fixed-time insemination after ovulation induction is commonly used in pigs to obtain in vivo produced embryos at defined stages of development for downstream biotechnological applications. Howe...
Recovery of Cryptosporidium from spiked water and stool samples measured by PCR and real time PCR
Cryptosporidium parvum oocysts spiked water and stool water filtration
2015/4/16
Cryptosporidium parvum is a common intestinal protozoan parasite infecting humans and a wide range of animals, whose diagnostics present considerable difficulties. These arise from the exceptionally r...
The effect of various forms of selenium supplied to pregnant goats on selected blood parameters and on the concentration of Se in urine and blood of kids at the time of weaning
ruminants Se-yeast sodium selenite Se-lactate Se-proteinate
2015/4/16
The aim of this trial was to compare the effect of supplementation of goats with different forms of selenium on the metabolism of their kids at the time of weaning. The experiment was performed with 4...
Mycobacterium avium subsp. paratuberculosis in powdered infant milk: F57 competitive real time PCR
Crohn's disease public health food safety environmental risk
2015/4/15
Mycobacterium avium subsp. paratuberculosis (MAP) in concentrations from 48 to 32 500 cells per gram of powdered infant milk were found in 18 out of 51 investigated samples (35%) in this study. More t...
Mycobacterium avium subsp. hominissuis infection in two sibling Fjord horses diagnosed using quantitative real time PCR: a case report
Mycobacterium avium complex mycobacteriosis qPCR IS1245 zoonosis
2015/4/15
This report describes new possibilities for intravital and post mortem diagnosis of avian mycobacteriosis in horses using the quantitative real time PCR (qPCR) method. Using this method, Mycobacterium...
Examination of Mycobacterium avium subsp. avium distribution in naturally infected hens by culture and triplex quantitative real time PCR
chicken mycobacteriosis non-tuberculous mycobacteria
2015/4/7
Mycobacterium avium subsp. avium (MAA) is the etiologic agent of avian tuberculosis, a chronic contagious disease described in a wide variety of domestic and wild bird species. The aims of this study ...
Mycobacterium avium subsp. paratuberculosis in a mouflon herd without clinical symptoms monitored using IS900 real-time PCR: a case report
MAP Johne's disease faeces
2015/4/7
The aim of this study was to monitor over two years a farmed mouflon herd for the presence and persistence of Mycobacterium avium subsp. paratuberculosis (MAP) using an IS900 real-time PCR method. Thi...
Determination of the proper time for mating after oestrous synchronisation during anoestrous or oestrous by measuring electrical resistance of cervical mucus in ewes
electrical resistance of cervical mucus oestradiol-17β
2015/5/25
In each case, one blood sample was collected from
all ewes at sponge insertion, at sponge removal and at oestrus, for the determination of serum οestradiol-17β and
progesterone concentrations, usi...
Real-time PCR for quantitation of bovine viral diarrhea virus RNA using SYBR Green I fluorimetry
bovine viral diarrhea virus RNA real-time RT-PCR SYBR Green I
2015/3/13
Quantitative real-time RT-PCR (qRT-PCR) assay was developed for the detection and quantification of bovine viral diarrhea virus (BVDV) in clinical samples from persistently infected cattle. qRT-PCR wa...
Comparison of immune parameters in cows with normal and prolonged involution time of uterus
dairy cows periparturient period phagocytes lymphocytes cell-mediated immune functions immu-nosuppression flow cytometry
2015/1/26
Indices of cellular immunity in postpartum Holsteincows with the normal (n = 9) and prolonged (n = 4) uterine involution time were evaluated. Peripheral white blood cells were isolated by lysis from p...
本研究旨在揭示感染JSRV病羊体内检测不到循环抗体的免疫学机理。用地高辛（DIG）标记制备enJSRV-env探针，原位杂交法检测妊娠70 d的绵羊胎儿（免疫器官初步形成期）、妊娠130 d胎儿（即将出生的）和出生7日龄羔羊的胸腺、脾脏、肠淋巴结和肺脏内enJSRV mRNA表达情况。并利用Real-Time PCR法，对enJSRV mRNA在以上组织中的表达进行定量分析。结果表明，在所检测组织...
Time Course Changes in 13C Enrichment of Respiratory CO2 in Chickens Intravenously Single-Injected with [1-13C] Sodium Acetate
oxidation intravenous acetate 13C enrichment chicks
2009/7/31
Time course changes in 13C enrichment of respiratory CO2 in young chickens intravenously injected with [1-13C] sodium acetate were examined to clarify the rapidity of oxidation of intravenous acetate...