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近日，由中国林业科学研究院林业研究所王军辉研究员主持完成的“一种基于NMR技术筛选粗枝云杉体胚萌发相关标记代谢物的方法”获国家发明专利授权。干化处理能显著提高粗枝云杉体细胞胚胎（体胚）萌发能力，但干化处理对粗枝云杉体胚内部代谢物水平的影响尚不清楚。王军辉研究员课题组基于粗枝云杉干化处理前后子叶和胚根代谢组结果分析，找到了一组与粗枝云杉体胚萌发密切相关的标记代谢物。本发明使用NMR技术检测了粗枝云杉...
为了发掘团头鲂对锌（Zn）敏感的代谢标志物，以3种Zn含量分别为7.4、32.1和332.4 mg/kg的半纯化饲料（分别标记为L、M和H组），喂养初始体质量为（3.6±0.1） g团头鲂12周，应用1H（氢谱）核磁共振波谱法（1H NMR）检测团头鲂血清代谢物的种类和浓度，再用最小二乘法判别（PLS-DA）和变量重要性（VIP）分析3个处理团头鲂血清的代谢轮廓和主要差异代谢物。结果显示，血清中5...
土壤磷形态组分分级及31P-NMR技术应用研究进展
土壤磷形态 化学连续提取法 土壤磷组分 31P-核磁共振波谱技术
2017/9/30
农田生态系统中土壤磷形态转化，影响土壤磷对作物的有效供应。土壤磷分为无机磷和有机磷两大部分。化学连续提取法（chemical sequential fractionation，CSF）研究土壤磷形态分级，采用不同的化学提取剂，分级提取土壤中组成或分解能力接近的有机无机含磷化合物，是目前表征土壤磷素形态的重要方法。但该方法虽历经改进，仍难以确切反映土壤磷的实际组成，提取的不同磷形态间存在重叠，有机磷...
为了从代谢组角度系统揭示奶牛分娩后能量缺乏的生理机制，采用1H-NMR 代谢组学技术，结合多元统计学方法探讨了奶牛分娩后不同阶段血浆小分子代谢物的变化趋势及其对能量代谢通路的影响。结果表明：奶牛分娩后第7天与分娩第1天之间血浆代谢轮廓无明显差异；分娩后第14天与第1天之间血浆代谢轮廓出现明显差异，其差异标志物为不饱和脂肪酸、胆碱和氧化三甲胺；分娩后第28天与第1天之间血浆代谢轮廓也存在明显差异，其...
低场NMR研究pH对肌原纤维蛋白热诱导凝胶的影响
猪肉肌原纤维蛋白凝胶 保水性 弛豫 pH
2009/8/31
【目的】探讨pH对猪肉肌原纤维蛋白热诱导凝胶保水性及水的移动性影响。【方法】从猪肉中提取肌原纤维蛋白,用低场NMR（nuclear magnetic resonance）研究pH对肌原纤维蛋白热诱导凝胶中水的T2弛豫性质的影响。同时用离心法测量pH对肌原纤维蛋白凝胶保水性（water holding capacity,WHC）影响。【结果】NMR结果拟合后得到水有4个组分,合并为对应水的3种状态即...
Tracing Effects of Na+ on Morphology, Phosphate Metabolism and Accumulated Compounds of a Sugar Beet Root by NMR
Beta vulgaris Daucus carota 1H-NMR imaging Salt stress
2009/7/31
Effects of Na+ on morphology, phosphate metabolism and accumulated compounds of sugar beet root were studied by 1H-NMR imaging, and 31P-, 23Na- and 13C-NMR. 23Na-NMR showed that Na+ concentration of ...
Cold- or Heat-Tolerance of Leaves and Roots in Perennial Ryegrass Determined by 1H-NMR
Arrhenius plots Cold stress Dynamic states of water Heat stress NMR relaxation times (T1,T2) Organ Perennial ryegrass Water compartment
2009/7/28
Dynamic states of water in the leaves and roots of perennial ryegrass (Lolium perenne L.) exposed to cold and heat stresses were studied by using 1H-NMR. NMR spin-lattice relaxation times (T1) of leav...
Changes in NMR Relaxation of Rice Grains, Kernel Quality and Physicochemical Properties in Response to a High Temperature after Flowering in Heat-Tolerant and Heat-Sensitive Rice Cultivars
‘Chikushi 64‘ High temperature Kernel quality ‘Nikomaru‘ NMR relaxation time (T1 and T2) Nucellar epidermis Rice Water status
2009/7/21
Rice productivity is related to the ability of plants to adapt to heat stress. The heat-tolerant cultivars ‘Nikomaru’ and ‘Chikushi 64’ and heat-sensitive cultivar ‘Hinohikari’ were grown at 30ºC...
Use of NMR for metabolic profiling in plant systems
NMR metabolic profiling plant metabolomics
2009/3/5
The review deals with the applications of solution state 1H NMR to the metabolic profiling of plant tissue extracts. NMR is introduced as one of several measurement techniques that are being used in...