搜索结果: 1-3 共查到“生物工程 Quantitative”相关记录3条 . 查询时间(0.031 秒)
Development and Application of a Mass Spectrometry-Based Quantitative Assay for Apolipoprotein M in Human and Mouse Serum
Mass Spectrometry Cholesterol Apolipoprotein M MS-Based Assay
2015/5/26
Apolipoprotein M (apoM) is necessary for the formation of lipid-poor preβ-HDL particles, the initial precursor of HDL and acceptors of cholesterol efflux from peripheral cells. An assay to quantify ap...
Connecting Motors and Membranes: A Quantitative Investigation of Dynein Pathway Components and in vitro Characterization of the NUM1 Coiled Coil Domain
Connecting Motors and Membranes Dynein Pathway Components vitro Characterization NUM1 Coiled Coil Domain
2014/12/12
In the budding yeast, Saccharomyces Cerevisiae, dynein, a minus-end directed motor, is involved in nuclear migration and proper orientation of the mitotic spindle during mitosis. Our lab has developed...
应用实时定量RT-PCR技术检测b地中海贫血珠蛋白基因的表达Quantitative Analysis of Human Globin Gene Expression in b-thalassemia Using Real-Time RT-PCR
地中海贫血/遗传学 珠蛋白基因 基因表达 实时定量RT-PCR
2008/1/6
摘要
为了定量检测 b 地中海贫血(b 地贫)的 a、b 和γ珠蛋白基因表达水平, 提取正常成人对照组、正常胎儿对照组和b 地贫患者组组成的样本 DNA,采用反向点杂交法(RDB)分析b 地贫各种突变类型;提取样本RNA用于进行针对a、b 和γ珠蛋白基因的荧光实时定量RT-PCR(FQ RT-PCR)。根据FQ RT-PCR原理,设计合成分别对应于a、b 和γ珠蛋白基因的3对引物和3条荧光探针,...