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Rapid adaptation in fruit flies（图）
果蝇 快速适应 进化周期
2023/7/11
Think evolution is a slow, gradual process? Tell that to fruit flies. In a new report published in Science, researchers from the University of Pennsylvania used a controlled field experiment to show t...
Molecular genetics of ecological diversification: Duplication and rapid evolution of toxin genes of the venomous gastropod Conus
ecological diversification Duplication
2015/12/17
Predatory snails in the marine gastropod
genus Conus stun prey by injecting a complex mixture of
peptide neurotoxins. These conotoxins are associated with
trophic diversification and block a divers...
一步3’RACE快速构建鸡MnSOD全长cDNA克隆Rapid Construction of Full-length MnSOD cDNA of Chickens by One-step 3’RACE
RACE 触减PCR MnSOD cDNA
2008/1/8
摘要
本研究尝试将触减 PCR与3’ cDNA末端快速扩增（rapid amplification of cDNA ends，RACE）技术进行结合，仅用一条特异性引物和一条通用引物，成功地实现了从3’末端cDNA库对鸡含锰超氧化物歧化酶（manganese-containing superoxide dismutase，MnSOD）全长cDNA的一步3’RACE快速构建。与常规使用的末端PCR...
cDNA末端快速扩增（RACE）技术是一种快速获得cDNA的3′和5＇端的方法。本文从RACE的原理出发，指出其技术本身存在的优缺点，阐述了RACE操作中须注意和不容忽视的技术要点，并对前人对RACE技术所做的改进加以总结，最后对RACE技术的应用前景给予了展望。Abstract: Rapid amplification of cDNA end (RACE) technique is a meth...