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Rice University scientists have effectively quenched a debate over the mechanism behind a fluorescent biosensor that monitors neurons by sensing changes in voltage.The work led by Rice theoretical che...
Yellow fluorescent protein (YFP) is widely used as a genetically encoded fluorescent marker in biology. In the course of a comprehensive study of this protein, we observed an unusual, negative fluores...
Green fluorescent proteins bearing the Y66H mutation exhibit strongly blue-shifted fluorescence excitation and emission spectra. However, these blue fluorescent proteins (BFPs) have lower quantum yiel...
Fluorescence interference contrast microscopy (FLIC) is a powerful method to structurally characterize fluorescent objects with nanometer-scale resolution in the z direction. Here we use FLIC to chara...
We have developed a single vesicle assay to study the mechanisms of supported bilayer formation. Fluorescently labeled, unilamellar vesicles (30-100 nm diameter) were first adsorbed to a quartz surfac...
Delayed fluorescence is observed from quinone-depleted Rhodobacter sphaeroides reaction centers due to re-formation of the excited state of the special pair electron donor from the initially formed ra...
The fluorescence intensity from randomly oriented Rhodobacter ( Rb.) sphaeroides photosynthetic reaction centers at 77 K increases in an applied electric field. This is ascribed to a net reduction in ...
During the course of DNA replication, replication forks often stall or collapse as they proceed from oriC to the terminus due to housekeeping types of DNA damage or proteins bound to DNA. If the DNA i...
Wavelet transform of polarized fluorescence spectra of human breast tissues is found to localize spectral features that can reliably differentiate normal and malignant tissue types. The intensity diff...
摘要正常对照标准值4.17%)。用FISH 技术检测性染色体数目异常克氏综合征具有快速、敏感度高、信号强、背景低、多色等优点,故FISH 技术在产前诊断检测领域中显示其重要的应用价值和发展前景。 Abstract:The objective of the work is to study the technique of dual-color fluorescence in situ hybri...
It has been known that the sex of chicken cells can be most accurately identified by fluorescence in situ hybridization (FISH). However, the presently available FISH has not been widely used for sex i...

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