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Terence D. Capellini has been interested in how joints work for almost three decades. Part of it is due to personal experience, as he suffered several joint injuries as a college ice hockey player and...
Researchers wirelessly manipulate gene, step toward reprogramming the human genome（图）
manipulate gene toward reprogramming human genome
2019/10/23
A new University at Buffalo-led study supported by NSF describes how researchers wirelessly controlled FGFR1 -- a gene that plays a key role in how animals grow from embryos to adults -- in lab-grown ...
Chemists Get Step Closer to Replicating Nature with Assembly of New 3D Structures（图）
chemists step closer replicating nature new 3d structures
2017/9/5
A team of New York University chemists has created a series of three-dimensional structures that take a step closer to resembling those found in nature. The work offers insights into how enzymes are p...
In a possible step forward for gene therapy,Stanford researchers made mice glow like fireflies
step forward gene therapy Stanford researchers mice glow fireflies
2017/3/14
Timothy Blake, a postdoctoral fellow in the Waymouth lab, was hard at work on a fantastical interdisciplinary experiment. He and his fellow researchers were refining compounds that would car...
Stem Cells Step Forward
Stem Cells Step Forward
2017/3/13
Researchers at Harvard Medical School and Boston Children’s Hospital were able, for the first time, to use patients’ own cells to create cells similar to those in bone marrow and then use them to iden...
COLUMBUS, Ohio—Researchers at The Ohio State University have gained new insight into how the body moves when we’re walking.
They learned everything they needed to know by watching people walk natural...
CORVALLIS, Ore. – Humans and some of our hominid ancestors such as Homo erectus have been walking for more than a million years, and researchers are close to figuring out how we do it.
The University of Queensland helps bring world-first Hendra treatment a step closer
Hendra treatment
2014/7/10
The University of Queensland is playing a part in a clinical trial of the only known effective treatment for humans exposed to Hendra virus.A joint grant of $1.2 million from the Queensland and NSW go...
Development of stem cell research for treating human cells damaged through injury, disease or ageing has taken a step forward in Queensland.
Analysis of noise-induced bistability in Michaelis Menten single-step enzymatic cycle
noise-induced bistability Michaelis Menten single-step enzymatic cycle
2011/8/11
Abstract: In this paper we study noise-induced bistability in a specific circuit with many biological implications, namely a single-step enzymatic cycle described by Michaelis Menten equations with qu...
Effects of Sucrose and Glycerol during the Freezing Step of Cryopreservation on the Viability of Goat Spermatozoa
Goat Spermatozoa Cryopreservation Osmolality Sucrose Glycerol
2016/4/28
Four experiments were conducted to study the following: i) the influence of different concentrations of sucrose (0.15, 0.3 and 0.5 M with osmolality of 308, 500 and 760 mOsm/kg, respectively) in dilue...
一步3’RACE快速构建鸡MnSOD全长cDNA克隆Rapid Construction of Full-length MnSOD cDNA of Chickens by One-step 3’RACE
RACE 触减PCR MnSOD cDNA
2008/1/8
摘要
本研究尝试将触减 PCR与3’ cDNA末端快速扩增（rapid amplification of cDNA ends，RACE）技术进行结合，仅用一条特异性引物和一条通用引物，成功地实现了从3’末端cDNA库对鸡含锰超氧化物歧化酶（manganese-containing superoxide dismutase，MnSOD）全长cDNA的一步3’RACE快速构建。与常规使用的末端PCR...
氯化钠密度梯度离心法制备用于显微注射的外源DNA片段Sodium Chloride Step Gradients Centrifuge to Fractionate Foreign DNA Fragment for Microinjection
显微注射 氯化钠密度梯度离心 胚胎存活率 外源基因整合率
2007/12/31
摘要DNA显微注射是生产转基因动物最可靠和最常使用的一种方法，外源DNA的纯度对显微注射的成功起着至关重要的作用。本文介绍用氯化钠密度梯度离心的方法制备用于显微注射的外源DNA片段。与传统的琼脂糖凝胶回收的方法相比较，用此方法制备的外源DNA片段对小鼠受精卵进行显微注射后，受卵体母鼠的胚胎存活率,以及子代小鼠的外源基因整合率均有明显的提高。这一方法可为进一步提高转基因动物的成功率,提供方法学上的参...
期刊信息
篇名
High-level expression and single-step purification of human tryptophenyl-tRNA synthetase
语种
英文
撰写或编译
撰写
作者
Xu Feng,Jia Jie,Jin You-xin(通讯作者),Wang De-bao
第一作者单位
中科院上海生化所分子生物学国家重点实验室
刊物名称
Protei...
期刊信息
篇名
High-level expression and single-step purification of leucyl-tRNA synthetase
语种
英文
撰写或编译
撰写
作者
Jian-feng Chen,En-Duo Wang(通讯作者),Ying-lai Wang
第一作者单位
中科院上海生化所分子生物学国家重点实验室
刊物名称
Protein Expressio...