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Probing the Structure of Supported Membranes and Tethered Oligonucleotides by Fluorescence Interference Contrast Microscopy
Water Oligonucleotides Microscopy, Fluorescence Microscopy, Interference Membranes, Artificial Sensitivity and Specificity Surface Properties Models, Chemical Silicon Dioxide
2016/5/24
Fluorescence interference contrast microscopy (FLIC) is a powerful method to structurally characterize fluorescent objects with nanometer-scale resolution in the z direction. Here we use FLIC to chara...
Probing Protein Electrostatics with a Synthetic Fluorescent Amino Acid
pteropod aragonite flux East Sea (Sea of Japan)
2016/5/24
Electrostatics affect virtually all aspects of protein structure and activity and are particularly important in proteins whose primary function is to stabilize charge. Here we introduce a fluorescent ...
Team Develops ‘Cool’ New Method for Probing How Molecules Fold
Cool New Method Molecules Fold
2014/12/30
Using the new system, researchers can force a sample of molecules to unfold and refold by boosting and then dropping the temperature, so quickly that even some of the fastest molecular folding events ...
A new strategy for RNA structure probing in vivo: Targeting methylation small nucleolar RNPs to new sites in ribosomal RNA
Eukaryotic ribosomal RNA nucleotide RNA proteins DNA methylation
2014/12/11
Eukaryotic ribosomal RNAs (rRNAs) contain scores of 2′-O-methylated nucleotides that are formed by a large family of small RNA:protein machines called snoRNPs. The snoRNPs contain a small nucleolar RN...
Probing The Regulation Of Vasculogenic Mimicry In Glioblastoma: Implications For Treatment In Patients
Health and environmental sciences Biological sciences Angiogenesis Anti-angiogenic treatment resistance Cancer Cancer stem cells Glioblastoma Vasculogenic mimicry
2014/12/10
Glioblastoma, previously known as Glioblastoma Multiforme (GBM), is a highly angiogenic tumor and is defined pathologically by its ability to create microvascular proliferations. Patients with gliobla...
Probing the Peptidyl Transferase Center of Ribosomes Containing Mutant 23S rRNA with Photoreactive tRNA
ribosome peptidyl transferase center photoreactive crosslinking tRNA 23S rRNA translation
2014/12/9
There is strong crystallographic evidence that the 23S rRNA is the only catalytic entity in the peptidyl transferase center. Various mechanisms for the catalysis of peptidyl transfer have been propose...
Probing for Conformational Changes in the Repair Enzyme Mfd Using Mutant Protein Constructs
Conformational Changes Repair Enzyme Mfd Mutant Protein Constructs
2014/12/9
DNA repair is essential for survival, as damage to the genome can interrupt the precarious balance of cell functions, causing further mutations and possibly leading to cancer. The bacterial transcript...