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With the rapid increase in production of genetic data from new sequencing technologies, a myriad of new ways to study genomic patterns in nonmodel organisms are currently possible. Because genome as...
Discovery laboratories are dealing with DNA sequencer-based technologies which have seen great advancement over the past decade, resulting in several steps of the genotyping process becoming automated...
Although more than 400 microsatellite loci are currently available for Vicia faba L. (faba bean), an important food and feed grain crop legume, they have not yet been used for comprehensive molecula...
The myostatin (MSTN) gene, as a negative regulator of skeletal muscle growth, has been proposed to be associated with production traits in farm animals. In the present study, a T/C variant at −1...
High-throughput genotype data can be used to identify genes important for local adaptation in wild populations, phenotypes in lab stocks, or disease-related traits in human medicine. Here we advance...
We have developed a simple, labor-saving, inexpensive, and rapid single nucleotide polymorphism (SNP) genotyping method that works directly on whole human blood. This single-tube genotyping method wa...
摘要 为研究采用PCR—SSP技术,建立可靠的人类血小板抗原HPA-1,2,3,4,5,6系统的同步基因分型方法,并以所建立的方法研究血小板抗原。设计合成18条序列特异性引物,探索最佳退火温度,通过调整引物浓度、Mg2+离子浓度,使HPA-1~6系统等位基因在同一条件下进行同步扩增和扩增产物在同一凝胶中进行同步电泳。引物的特异性和灵敏度采用基因型已知的质控DNA进行验证。应用此方法,对2000年...
摘要选用18对大熊猫特异的微卫星引物对成都动物园、成都大熊猫繁育研究基地的大熊猫幼子及其母亲和可疑父亲DNA作了PCR扩增。扩增产物在ABI 373测序仪上做基因扫描。 应用Genotyper 672 软件处理分型数据,经Cervus 2.0 软件对亲子关系进行分析。结果表明, 在母子关系确实的情况下,18个基因座联合的父亲鉴定排除概率E为0.999921,置信度为95%。而在母子关系不清的条件下...
Three buffalo populations viz. Bhadawari, Tarai and local buffaloes of Kerala were genotyped using 24 heterologous polymorphic microsatellite loci. A total of 140 alleles were observed with an average...
We have developed a reliable and noninvasive method for swine genotyping of single locus nuclear gene with aged single hair follicles delivered by general mail. The method is based on booster and nest...

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