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A two Compartment microdialysis microdevice for continuous protein extraction from whole blood
microdialysis cardiopulmonary bypass anaphylatoxin
2009/12/24
This work presents the design of a continuous microdialysis based blood protein
extraction system to be used for clinical monitoring of inflammatory responses in
patients undergoing cardiac surgery....
Development of a microfluidic module for DNA purification from bacterial lysates via phenol extraction
DNA Purification Phenol Extraction Multi-Phase Microfluidics
2009/12/24
In this work microfluidic platforms designed to miniaturize the phenol extraction
technique commonly used to purify DNA from cell lysates have been developed.
First, a long serpentine device was use...
A Simple Stabilization Method of Reduced Albumin in Blood and Plasma for the Reduced/Oxidized Albumin Ratio Measurement
reduced albumin human serum albumin stabilization method mercaptalbumin mass spectrometry
2009/10/30
Albumin (Alb) is mixture of reduced and oxidized forms. It is physiologically significant to determine Alb(red)%, which is the proportion of reduced Alb in the sum of Alb. However, reduced Alb in both...
采用DNA和蛋白质吸收带的Kubelka-Munk光谱函数对离体的人正常胃、未分化胃腺癌、胃鳞状细胞癌和低分化胃腺癌的上皮组织进行鉴别诊断。实验采用带积分球附件的分光光度计获取组织的漫反射光谱。结果表明：在250～650 nm，人胃的上皮组织的癌变导致未分化胃腺癌、胃鳞状细胞癌和低分化胃腺癌等的上皮组织的Kubelka-Munk光谱函数f(r∞)及其对数log[f(r∞)]的平均值在DNA吸收带(...
近红外光谱监测体外循环手术中脑组织氧合状况的研究
体外循环 近红外光谱 局部脑组织氧饱和度
2009/10/29
体外循环手术中，为防止因脑氧供需失衡导致脑缺氧，就要实时监测患者局部脑组织的氧合状况，以根据其变化调整生理参数或采取应急手段。用该研究小组自行研制的近红外仪器(使用一个双波长的近红外光源和两个近红外检测器)监测心脏手术中患者的脑氧，可以求出局部脑组织血红蛋白浓度的变化，并根据稳态空间分辨光谱(SRS)算法求出局部脑组织的氧饱和度(rSO2)。体外循环中用监护仪监测患者的混合静脉血氧饱和度(SvO2...
离体正常乳腺组织350～850 nm波段光谱特性
乳腺组织 光谱特性 反向倍加法
2009/10/28
采用带有积分球附件的紫外/可见/近红外分光光度计测量了离体正常乳腺组织在350～850 nm光谱范围的反射率和透射率，运用反向倍加法得到了离体正常乳腺组织在相应光谱范围的光学参数，分析了正常乳腺组织的光学穿透深度随波长的变化情况。实验结果表明：350～850 nm波段正常乳腺组织的约化散射系数μ′s大于吸收系数μa。μ′s随着波长的增加而减小，即从350 nm波长值为9.731 mm-1～850 ...
脉冲微波辐照影响心肌细胞膜蛋白构象及其机制的研究
傅里叶变换红外光谱 脉冲微波 心肌细胞
2009/10/28
应用显微傅里叶变换红外光谱技术研究了脉冲微波辐照对心肌细胞膜蛋白质构象、功能的影响和相关分子机制。结果表明，辐照可对心肌细胞的细胞膜蛋白质结构产生明显影响。细胞膜脂质中—CH2—、磷脂结构中CO、蛋白质酰胺Ⅰ，Ⅱ带的伸缩振动峰消失或位移。辐照后心肌细胞膜蛋白质二级结构也出现明显变化，α-螺旋和β-折叠结构减少，二级结构无序化程度增加。上述变化均与辐照剂量呈正相关。结果提示受脉冲微波辐照后，心肌细胞...
泌尿系结石组分分析方法及其研究进展
泌尿系结石 组分分析 仪器分析
2009/10/28
对泌尿系结石组分进行准确的分析可为治疗尿石症和预防其复发提供重要的参考。文章综述了现代仪器分析方法在草酸钙结石、磷酸盐结石、尿酸和尿酸盐结石及胱氨酸类结石等组分分析中的应用及其研究进展，这些技术包括拉曼光谱、差热-热重(TGA/DTA)、核磁共振(NMR)、高效液相色谱(HPLC)和傅里叶红外光谱(FTIR)等。
现代仪器在泌尿系结石元素分析中的运用
仪器分析 泌尿系结石 元素分析
2009/10/28
对泌尿系结石所含元素进行准确的分析可为治疗尿石症和预防其复发提供重要的参考，文章综述了现代仪器分析技术在泌尿系结石元素分析中的运用及其研究进展，这些技术包括：X射线光电子能谱(XPS)、质子激发X射线发射光谱(PIXE)、能量分散X射线分析(EDX)、电子束探针微区分析(EPMA)、原子发射光谱(AES)、原子吸收光谱(AAS)、电感耦合高频等离子体发射光谱(ICP)、X射线荧光光谱(XRF)及离...
消除个体条件测量差异的动态光谱及其频域提取法的研究
傅里叶变换 近红外光谱 无创检测
2009/10/28
在近红外光谱的皮肤无创检测中，个体差异是一个相当大的技术难题。在血液成分无创检测中，个体差异包括毛发、角质层、表皮、真皮、皮下组织、肌肉、骨骼等。研究表明，个体差异随着个体不同而不同，是阻碍血液成分无创检测技术实用化的重要因素。文章提出了基于光电容积脉搏波的产生机理和傅里叶变换的检测血液成分浓度的新方法——频域提取法，这种方法可以消除测量中由于皮肤组织和肌肉组织产生的差异;提出了动态光谱的概念，从...
Spectrophotometric, Spectrofluorimetric and Voltammetric Analyses of Naftidrofuryl Oxalate in its Tablets
naftidrofuryl oxalate derivative spectrophotometry spectrofluorimetry differential-pulse voltammetry pharmaceutical tablets
2009/10/26
This work deals with several direct and indirect spectrophotometric, spectrofluorimetric and voltammetric analyses of the vasodilator drug: naftidrofuryl oxalate (NF). For the derivative spectrophotom...
Spectrophotometric Methods for the Determination of Cefprozil in Bulk and Dosage Form
determination spectrophotometry liquid chromatography ascorbic acid sodium hydroxide kinetics cefprozil tablets
2009/10/26
Two simple spectrophotometric methods were developed for the determination of cefprozil in pure bulk and in tablets forms. The first is a colorimetric method based on the coupling of cefprozil, after ...
Spectrophotometric Determination of Labetalol and Lercanidipine in Pure Form and in Pharmaceutical Preparations Using Ferric-1,10-Phenanthroline
Ferric-1 10-Phenanthroline labetalol lercanidipine spectrophotometry
2009/10/26
A simple and sensitive spectrophotometric method was developed for the determination of labetalol HCl (LBT) and lercanidipine HCl (LER) in pure form and in dosage forms. The method was based upon oxid...
Automatic noninvasive measurement of systolic blood pressure using photoplethysmography
Automatic noninvasive measurement systolic blood pressure photoplethysmography
2010/2/25
In this study, we present a cuff-based technique for automatic measurement of systolic blood pressure, based on photoplethysmographic signals measured simultaneously in fingers of both hands. After in...