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为明确侧孢短芽孢杆菌BL-21(Brevibacillus laterosporus)产生的抗菌物质的类型,对其发酵液抗菌活性的稳定性进行分析,并在此基础上对有效成分进行分离纯化。采用杯碟法检测了B laterosporus BL-21代谢产物的热稳定性、酸碱稳定性和蛋白酶稳定性;通过硫酸铵盐析、CM-Sepharose Fast Flow离子交换层析、凝胶过滤层析和SDS-PAGE进行了分离纯化...
基于cDNA末端快速扩增(RACE)技术克隆了两个糙皮侧耳凝集素基因Plectin1和Plectin2。结构分析显示Plectin1和Plectin2基因全长分别为1 371和1 359 bp,二者均含有5个外显子和4个内含子;Plectin1开放阅读框长1 134 bp,编码377个氨基酸,Plectin2开放阅读框长1 122 bp,编码373个氨基酸。Southern杂交试验证实Plecti...
目的:探讨杂粮蛹虫草菌丝共生体中虫草素的最优提取条件,并比较了不同光照时长对虫草素含量的影响,为其工艺条件的优化和综合利用提供依据。方法:采用单因素结合响应曲面法优化虫草素的水浴提取条件,高效液相色谱法测定虫草素含量。结果:最优水浴提取条件为:浸提时间6.59 h、浸提温度81.9℃、料液比1∶90,此条件下虫草素含量为6.0143 mg/g;不同光照时长条件下虫草素含量为3.8988~11.96...
Th e defensive reaction of apple cultivar Idared (Malus domestica Borkh.) was studied after inoculation with three diff erent pathogens (Penicillium expansum, Monilinia fructigena, and Gloeospor...
Our biotechnological research on selected Cucumis species has encompassed interspecific hybridization via embryorescue, in vitro pollination, somatic hybridization and cytogenetics of protoplasts. Emb...
The goal of this study was to determine an efficient micropropagation system for cranberry (Vaccinium macrocarpon Ait.). Cranberry cultivar Howes was successfully established in vitro using mercuric...
This paper deals with the results of studies on changes in the content of total anthocyanins depending on their fermentative maceration from grapes of traditional Moravian cultivars (Blauer Portugiese...
In vitro selected salt tolerant saplings of Morus alba (cv. Sujanpuri) were raised from nodal explants with axillary buds collected during three different periods of the year. The growth and shoot/roo...
Thymbra spicata var. spicata, which grows wildly in the flora of Turkey, is known as thyme. A field trial was conducted to determine suitable harvesting time and cutting height for Thymbra, therefore ...
The effect of two commercially produced biopreparations on the originators of chestnut ink disease in Slovakia – soil fungi Phytophthora cambivora and Phytophthora cinnamomi was tested in laboratory c...
Vegetable juices processed by lactic acid fermentation bring about a change in the beverage assortment for their high nutritive value, high content of vitamins and minerals. Starter cultures of the ge...
食用菌(Edible mushroom)由于其独特的风味和鲜嫩的肉质,在很多国家都被誉为佳肴。野生食用菌富含多糖、维生素和矿物质等,具有抗肿瘤和调节免疫等多种功能,是世界公认的不可多得的高营养兼药膳的"素中之荤"。和其他食物相比,野生食用菌富集重金属的能力强,由于矿物质和重金属会对人体产生积极和消极两种效应,近年来关于食用菌中矿物质和重金属含量的研究逐渐增加。本文对近年来野生食用菌的矿物质和重金属...
微量元素是一类人体所必需的营养素,在自然界中多数以无机态形式存在,且分布不平衡,不易被人体吸收利用。近年来许多研究显示,食用菌对微量元素锌、硒、碘、铁等都具有富集和将无机态微量元素转化成有机态的功能。本文对食用菌富集微量元素的机理进行了归纳总结,概述了灵芝、香菇和金针菇等各类食用菌对微量元素的富集作用的研究进展。
Olive trees are often subjected to a long dry season with low water availability which induces oxidative stress. The present study was conducted to evaluate the effectiveness of native Rhizophagus man...
以杏鲍菇为主要原料,添加适宜辅料,将其加工成类似火腿肠的凝胶食品。通过单因素和正交实验,研究了大豆蛋白、玉米淀粉、卡拉胶添加量对杏鲍菇肠品质特性的影响,在此基础上探讨了肉的种类和添加量对菇肠品质的影响。结果显示,以100g杏鲍菇浆料为基准,添加15%大豆蛋白、12%玉米淀粉、0.4%卡拉胶,杏鲍菇肠品质较好;添加30%鸡肉或猪肉,可进一步改善产品品质。扫描电镜观测显示,杏鲍菇肠内部呈现良好的空间网...

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