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A simple method for assessing hyaluronic acid production by cumulus-oocyte complexes
oocyte meiotic maturation cumulus expansion
2016/7/6
The cumulus expansion of cumulus-oocyte complex (COC) is an essential regulating process of oocyte maturation and as such it is a possible biomarker of the in vitro maturing oocytes quality. Cumulus e...
Male pronucleus formation after ICSI: effect of oocyte cysteine or sperm Triton X-100 treatments
porcine assisted reproductive technology
2015/7/7
While no cleavage was observed in the
CYS group, no significant differences in the cleavage rate were observed between control (21.3%) and TX (10.5%)
groups. In summary, and under our conditions, ...
Inhibition of c-Jun N-terminal kinase (JNK) suppresses porcine oocyte ageing in vitro
MAPK DNA fragmentation apoptosis Bax
2015/6/24
Oocyte ageing is a complex of processes that occur when matured in vitro oocytes are, after reaching the metaphase II stage, exposed to further in vitro culture. Aged oocytes remaining at the metaphas...
Expression and localization of nitric oxide synthase isoforms during porcine oocyte growth and acquisition of meiotic competence
pig oocyte growth meiotic competence
2015/6/8
Reproduction biotechnologies depend on the use of fully meiotically competent oocytes. Growing oocytes without full meiotic competence are an interesting potential source due to their quantity, but th...
Superovulation following follicular synchronization with GnRH at random stages of the oestrous cycle in heifers: oocyte competence and in vitro embryo production
heifer oocyte GnRH oocyte competence
2015/6/8
The objective of this study was to develop a superovulatory program based on the synchronization of follicular waves with GnRH which could be applied regardless of the stage of the oestrous cycle. In ...
The influence of cadmium on Prussian carp oocyte maturation, development of eggs and hatching
heavy metals oocyte maturation steroid hormones embryonic development hatching enzyme
2015/6/5
The influence of cadmium (Cd) on the last stage of Prussian carp (Carassius auratus gibelio B.) oocyte maturation and embryonic development was investigated. The (postvitellogenic) oocytes were incuba...
Effects of oocyte collection techniques and maturation media on in vitro maturation and subsequent embryo development in Boer goat
Boer goat collection techniques oocyte maturation ovary medium
2015/6/4
The oocytes (experiment 1) were harvested by one of the four collection techniques (slicing, puncture, aspiration I and aspiration II) and the total number and the number of each grade of oocytes were...
The invasive and new non-invasive methods of mammalian oocyte and embryo quality assessment: a review
oocyte embryo quality microfluidics
2015/4/16
The quality of oocytes-embryos can be determined by several techniques, including morphological, molecular, cellular and biochemical ones. The morphological methods of female gamete or embryo quality ...
Selected molecular and microfluidic aspects of mammalian oocyte maturation-perspectives: a review
microfluidics Lab-on-chip oocyte maturation media supplementation
2015/4/15
Maturation of oocytes is the most important stage, which determines the subsequent successful fertilization, zygote formation, attainment of blastocyst stage, normal embryo growth and development, as ...
Effect of acrosome reaction progress in frozen-thawed boar spermatozoa on the efficiency of in vitro oocyte fertilization
pig cryopreserved sperm assessment
2015/4/7
A good functional status of cryopreserved boar spermatozoa is very important for successful fertilization of porcine oocytes and in vitro embryo production. The purpose of the study was to evaluate th...
Assessment of transcript and protein levels contributing to cell cycle control and gap junction connections in morphologically variable groups of porcine cumulus-oocyte complexes
pig oocyte connexins
2015/4/7
Oocytes and somatic cumulus cells are connected by an extensive network of gap junctions. These connections contribute in a major way to oocyte maturation and developmental competence. Cumulus-oocyte ...
Induction of final oocyte maturation in Cyprinidae fish by hypothalamic factors: a review
reproductive dysfunction ovulation luteinizing hormone
2015/4/2
Gonadotropin-releasing hormone in Cyprinidae as in other Vertebrates functions as a brain signal which stimulates the secretion of luteinizing hormone from the pituitary gland. Two forms of gonadotrop...
Vitrification of immature bubaline cumulus oocyte complexes by the open-pulled straw and conventional straw methods and their subsequent in vitro fertilization
buffalo in vitro fertilization (IVF) oocytes
2015/3/17
The in vitro maturation (IVM), fertilization (IVF) and morphological changes in buffalo cumulus oocyte complexes (COCs) cryopreserved by ultra rapid freezing using conventional (CON) and open-pulled
...
Characterizing the Heavy Metal Chelator, TPEN, as a Ca2+ Tool in the Mammalian Oocyte
Calcium Oocyte TPEN Endoplasmic Reticulum Egg
2014/12/12
N,N,N’,N’-tetrakis-(2-Pyridylmethyl) ethylenediamine (TPEN) is a heavy metal chelator with high affinity for zinc. TPEN causes important responses in mammalian eggs. For example, these eggs are arrest...
CHARACTERIZATION OF Ca2+ INFLUX PATHWAY(S) DURING MOUSE OOCYTE MATURATION
Calcium influx oocyte maturation SOCE Stim1 Phosphorylation
2014/12/10
Ca2+ signaling induced at fertilization, also known as oscillations, is essential in mammalian eggs to initiate early embryonic development. The generation of the oscillations relies on optimization o...