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Evaluation of oxidative DNA damage in human sperm and its association with male infertility
8-Hydroxydeoxyguanosine spermatozoa infertility
2009/2/18
Recently, there is increasing evidence suggesting that oxidative sperm DNA damage is closely associated with impaired sperm function and male infertility. 8-Hydroxydeoxyguanosine (8-OHdG) is consi...
An immunochemical assay to detect DNA damage in bovine sperm
immunochemical assay DNA damage bovine sperm
2008/12/25
An immunochemical assay has been developed to detect oxidative damage in bovine sperm DNA. Sperm DNA contains a large amount of oxidative damage as a result of exposure to exogenous agents, but dama...
DNA Damage in Bovine Sperm Does Not Block Fertilization and Early Embryonic Development But Induces Apoptosis After the First Cleavages
Mitotic spindles blastocyst ICSI
2008/12/23
The main goal of this study was to investigate whether and at what level damage of paternal DNA influences fertilization of oocytes and early embryonic development. We hypothesized that posttesticul...
Nickel-Induced Oxidative Stress in Testis of Mice：Evidence of DNA Damage and Genotoxic Effects
Nickel chloride oxidative damage apoptosis abnormal sperms dominant lethal mutations
2008/12/22
Oxidative stress (OS) mechanisms are speculated to play a significant role in nickel-induced toxic effects and their carcinogenic potency. Although nickel-induced oxidative damage in somatic tissues...
Spermiogenic Germ Cell Phase–Specific DNA Damage Following Cyclophosphamide Exposure
Sperm comet assay chromatin remodeling susceptibility toxicology
2008/12/22
The production of genetically competent spermatozoa is essential for normal embryo development. The chemotherapeutic drug cyclophosphamide creates cross-links and DNA strand breaks in many cell type...
Cryopreservation of Ram Semen Facilitates Sperm DNA Damage: Relationship Between Sperm Andrological Parameters and the Sperm Chromatin Structure Assay
Artificial insemination semen quality motility chlortetracycline viability SCSA
2008/12/22
We hypothesized that cryopreservation and incubation in conditions that mimic the female genital tract following insemination increases the susceptibility of ram sperm DNA to denaturation. Ram spe...
Detection of DNA damage in response to cooling injury in equine spermatozoa using single-cell gel electrophoresis
DNA damage equine spermatozoa single-cell gel electrophoresis
2008/12/1
Single-cell gel electrophoresis (SCGE), or comet assay, has the ability to detect damage at the single cell level and has not been reported for equine sperm. The ability to detect nuclear damage at ...