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白纹伊蚊AGO2和Dcr-2基因片段克隆及各发育期转录水平分析
白纹伊 RNA干扰 AGO2 Dcr-2
2012/7/10
克隆白纹伊蚊（Aedes albopictus）RNA干扰通路相关AGO2和Dcr-2基因片段，并分析该蚊种不同发育阶段这2个基因片段的转录水平。 方法 根据埃及伊蚊（Ae. aegypti）AGO2和Dcr-2蛋白的氨基酸序列保守区设计简并引物，经RT-PCR从白纹伊蚊雌蚊总RNA中分别扩增AGO2和Dcr-2 cDNA，并与载体pMD18-T连接，转染大肠埃希菌（E. coli）后，筛选阳性...
Assessment of use of DcR 3 in diagnosis of dysplastic lesions and adenocarcinoma of the esophagus
Esophageal adenocarcinoma Barrett esophagus
2010/4/27
Background: Because of confusion to gastric cancers arising at the gastro-esophageal junction, true esophageal adenocarcinoma was thought to be unusual. Esophageal adenocarcinoma (EAC) is becoming mor...