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Development of Polymerase Chain Reaction assays with host-specific internal controls for Chlamydophila abortus
Chlamydophila abortus internal control PCR
2015/5/25
Therefore, culture-independent detection methods such as the polymerase chain reaction
are increasingly important and needed. The aim of this study was to develop a polymerase chain reaction assay
...
Development of Polymerase Chain Reaction assays with host-specific internal controls for Chlamydophila abortus
Chlamydophila abortus internal control PCR
2015/4/30
Chlamydophila abortus (C. abortus) is one of the most important infectious agents causing abortion in ruminants. The bacterium is obligately intracellular, cannot grow on agar, but it needs cell cultu...
Unusual fatal avian polyomavirus infection in nestling cockatiels (Nymphicus hollandicus) detected by nested polymerase chain reaction
budgerigar fledgling disease Melopsittacus undulatus parrots viruses PCR
2015/3/13
High mortality of nestling cockatiels (Nymphicus hollandicus) was observed in one breeding flock in Slovakia. The nestling mortality affected 50% of all breeding pairs. In general, all the nestlings i...
Polymerase chain reaction assay for the diagnosis of experimentally infected pregnant Sprague-Dawley rats with Brucella abortus biotype 1
Sprague-Dawley rats polymerase chain reaction South Korea
2015/2/5
In order to diagnose the experimentally infected pregnant Sprague-Dawley (SD) rats with Brucella abortus biotype 1 using polymerase chain reaction (PCR) assay, the SD rats were injected subcutaneously...
Detection the 4/91 strain of infectious bronchitis virus in testicular tissue from experimentally infected rooster by reverse transcription-polymerase chain reaction
Infectious bronchitis virus testicular tissue rooster
2010/9/13
Avian infectious bronchitis (IB) was initially considered to be a disease primarily of the respiratory and female reproductive tract of chicken. Avian infectious bronchitis virus has wide and variable...
New Screening Test for Male Germline Chimeric Chickens by Polymerase Chain Reaction Using Shingle Nucleotide Polymorphism Detection Primers
chimeric chicken germline mitochondrial DNA D-loop region single nucleotide polymorphism sperm
2009/7/31
Since it is impossible to distinguish germline chimeric chickens by appearance only, a progeny test is required for analyzing germline chimerism. To reduce the labor, time and expense involved in such...