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山羊卵巢维持基因FOXL2启动子活性及调控区域分析
山羊 FOXL2基因 启动子 活性
2015/12/30
旨在研究山羊卵巢维持基因FOXL2启动子活性以及探究该基因的调控机理。从NCBI数据库调取FOXL2基因启动子序列,用生物信息学软件对其核心启动子和转录因子进行预测分析。使用 PCR技术克隆FOXL2基因启动子序列,并构建一系列缺失载体,瞬时转染293T和A375细胞,利用双荧光素酶基因检测仪测定相对荧光素酶活性值。结果表明,该基因启动子区域存在两个典型的CpG岛,分别位于(-920/+51(97...
Profiles of mRNA Expression of FOXL2,P450arom,DMRT1,AMH,P450c17,SF1,ERα and AR,in Relation to Gonadal Sex Differentiation in Duck Embryo
dmrt1 duck gonad foxl2 mRNA P450arom
2009/7/30
The present study was conducted to show a profile of mRNA expression of sex-differentiation related genes in the duck gonad. Fertile duck eggs (Cherry-valley) were incubated (hatching at day 28 of inc...