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Identification of Transferred Chicken Germ Cells in Quail Gonad and Semen by Amplification of Chicken-Specific PCR Products
microsatellite DNA chicken PCR primordial germ cells quail
2009/8/3
A polymerase chain reaction (PCR) primer set for a chicken microsatellite locus on the Z chromosome, LEI0171 (GenBank Accession No.X85538), amplified a 363bp fragment of genomic DNA in chickens (Gallu...
Restoration of Genetic Resources from Ehime Native Chicken via Transferred Primordial Germ Cells (PGCs)
chimeras germ line primordial germ cell embryos
2009/8/3
This experiment was designed to produce chimeric chicken by transferring primordial germ cells (PGCs) of Ehime native chicken embryos to White Leghorn ones. Fertilized eggs produced by both chicken br...
Population of Circulating Primordial Germ Cells in Early Japanese Quail Embryos
primordial germ cells circulation erythrocytes quail embryo
2009/8/3
Avian primordial germ cells (PGCs) show a unique migration pathway during early development. As soon as the blood vessels have formed, PGCs enter the circulatory system, and migrate to the gonadal pri...
In vitro Proliferation of Chick Primordial Germ Cells Co-cultured with Germinal Ridge Stroma Cells
chicken PGCs in vitro culture proliferation germinal ridge stroma cells
2009/8/3
In order to develop the technique for in vitro culture of chicken primordial germ cells obtained from circulating embryonic blood vessels (cPGCs), participation of feeder cells and some of growth fact...
Developmental Genetic Analysis of the Avian Primordial Germ Cells and the Applications for Production of Chimeric Chickens
Germ Cells Chimeric Chickens Avian
2009/7/31
Developmental Genetic Analysis of the Avian Primordial Germ Cells and the Applications for Production of Chimeric Chickens.
Genetic Factors Affect the Number of Circulating Primordial Germ Cells in Early Chick Embryos
primordial germ cells circulation chick embryo male female
2009/7/31
Avian primordial germ cells (PGCs) arise from the epiblast and circulate temporarily via the blood vascular system in their migration route to the gonadal anlage. There are egg-to-egg variations in th...
Effects of Soft X-Ray Irradiation to Stage X Blastoderm on Restriction of Proliferation of Primordial Germ Cells in Early Chicken Embryos
chick embryo primordial germ cell soft X-ray stage X blastoderm
2009/7/30
It is necessary to deplete the endogenous primordial germ cells (PGCs) from recipient embryos when germline chimaeric chickens are produced by the transfer of PGCs. In this study, effects of soft X-ra...
Intense Expression of GFP Gene in Gonads of Chicken Embryos by Transfecting Circulating Primordial Germ Cells in vitro and in vivo
gonads green fluorescent protein primordial germ cell transfection
2009/7/30
The present study was carried out to develop techniques for introducing exogenous DNA into primordial germ cells (PGCs) and expressing the introduced DNA efficiently in the gonads of developing chicke...
Effect of Transfer Primordial Germ Cells (PGCs) into Chick Gonad
chicken embryo gonad PGCs transfer
2009/7/30
Primordial germ cells (PGCs) of white leghorn Maria line hens embryos as a donor were transferred white leghorn Laura line hens embryos as a recipient. During stage 12 to 15 of incubating fertilized ...