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Effects of erythromycin on milk yield, rumen contraction rate and blood beta-hydroxybutyrate concentration in dairy cows after laparoscopic reposition of the left displaced abomasum
cattle dislocatio abomasi endoscopy prokinetic drugs gastrointestinal mobility
2016/9/1
The objective of this study was to evaluate the beneficial effects of erythromycin (Emy), a prokinetic drug active at the level of the abomasum, duodenum and proximal duodenum, in dairy cows undergoin...
牛偏爱密码子优化后的Beta蛋白多克隆抗体制备
原核表达 蛋白纯化 多克隆抗体
2015/12/2
本研究旨在优化λ噬菌体bet基因，使其能够在牛细胞中高效表达，通过密码子优化及全基因合成方法制备牛bet基因，并进行原核表达和多克隆抗体制备及抗体效价评估。从GenBank中获得λ噬菌体bet基因的序列，登录号为AP005153，用牛偏爱密码子对其进行优化并将其合成。根据优化后的基因序列设计引物，用PCR扩增优化后的bet基因，随后将其克隆到pET28原核表达载体，并转化入大肠杆菌BL21中进行诱...
Opium alkaloids counterparts are secreted by human and animal organisms but the role of endogenous opioid peptides in horses has not yet been fully elucidated. Endogenous opioids are involved in regul...
ESBL and AmpC beta-lactamase-producing Enterobacteriaceae in poultry in the Czech Republic
Escherichia coli ESBL AmpC
2015/4/8
A major reason for resistance of Enterobacteriaceae to beta-lactam antibiotics is production of ESBLs and AmpC beta-lactamases. As their more detailed description in poultry is unavailable in the Czec...
The morphology of porcine oocytes is associated with zona pellucida glycoprotein 3 and integrin beta 2 protein levels--
pig oocyte morphology
2015/4/8
The morphology and quality of oocytes is set during oo- and folliculo-genesis, and is completed during final maturation. Early embryonic development is associated with the morphology of the cumulus-oo...
Antibody responses in buffalos immunized with Clostridium perfringens beta and epsilon toxoids
Clostridium perfringens antibody titre toxoid buffalo
2015/1/20
The antibody responses to toxoids were measured to investigate whether Clostridium perfringens beta and epsilon toxoids induced protective humoral immune responses in buffalos. Total of 24 buffalos we...
Changes in serum concentration of 17 beta-estradiol in female rats during estrous cycle after treatment with atrazine and zearalenone
atrazine zearalenone estrous cycle
2015/1/20
A daily dose of 14 mg atrazine and 2.5 mg zearalenone, given p.o. during 5 days of estrous cycle to female rats, changed their estrous cycle in comparison with control animals. On day –1 of expected e...
Rat Cardiomyocytes Express a Classical Epithelial Beta-Defensin
Innate immunity host defense peptides heart microdissection
2009/1/19
Beta-defensins (BDs) are classical epithelial antimicrobial peptides of immediate importance in innate host defense. Since recent studies have suggested that certain BDs are also expressed in non-trad...