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旨在研究促卵泡素（FSH）对牦牛卵母细胞体外成熟，以及对表皮生长因子（EGF）、表皮生长因子受体（EGFR）的表达影响和与细胞凋亡的关系。在牦牛卵母细胞体外成熟培养液中添加不同浓度FSH （终浓度分别为0、2、5、10 μg·mL-1），体外成熟培养后，运用实时荧光定量PCR （qRT-RCR）和免疫荧光染色技术检测EGF、EGFR表达情况，采用qRT-RCR和蛋白免疫印迹法检测Bax、Bcl-2...
As an independent non-profit membership organization EGF actively links all associations, institutions and individuals interested in grassland issues from more than 30 European member countries and th...
Improvement of developmental competence of aged porcine oocytes by means of the synergistic effect of insulin-like growth factor-1 (IGF-1) and epidermal growth factor (EGF)
epidermal growth factor insulin-like growth factor 1 oocyte ageing pig
2015/6/1
There is a constant increase in demand for large numbers of high-quality oocytes of domestic mammals for various reproductive biotechnologies. Prolonged in vitro culture represents one of the many pos...