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抗体夹心BGT-ELISA方法的建立及在转基因白桦中的应用
转基因白桦 多克隆抗体 酶标抗体 ELISA
2010/2/21
本文以转基因白桦（Betula platyphylla Suk.）中蜘蛛杀虫肽与Bt-toxin C肽融合蛋白（BGT）基因的表达产物为研究目标，采用原核表达及纯化出融合蛋白His-BGT作为抗原进行兔免疫，得到相应的抗血清，采用ELISA法检测效价在1:10 000以上，并对抗血清进行了免疫亲和层析，获得了高纯度的IgG，Western blot检测具有较好的特异性。采用过碘酸钠法将抗体标记辣根...
BORRELIA BURGDORFERI INFECTION AMONG FORESTRY WORKERS - ASSESSED WITH AN IMMUNOENZYMATIC METHOD (ELISA), PCR, AND CORRELATED WITH THE CLINICAL STATE OF THE PATIENTS
borreliosis forestry workers serology ELISA
2010/12/24
Occurrence of borreliosis in human population is associated with possibility of contact with the biological vector of this disease - a common European tick, Ixodes ricinus.