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Studies on cell wall regeneration in protoplast culture of legumes – the effect of organic medium additives on cell wall components
arabinogalactan proteins cell wall cellulose chitosan Fabaceae protoplast culture
2014/7/9
The cell wall regeneration in mesophyll protoplasts of yellow lupin and grass pea was studied. The occurrence of cell wall components: cellulose, callose and arabinogalactan proteins was analysed duri...
Optimisation of protoplast isolation protocols using in vitro leaves of Dendrobium crumenatum (pigeon orchid)
Dendrobium crumenatum cellulase pectinase protoplast sorbitol
2011/1/12
Protoplast isolation from in vitro leaves of Dendrobium crumenatum was carried out. Factors affecting protoplast isolation, sorbitol concentration, enzyme combinations and concentration and incubation...
Isolation and Culture of Protoplast from Finger Millet(Eleusine coracana)Callus
Cell division Cell wall Finger millet Protoplast isolation and culture
2009/8/5
High yield of protoplast was obtained from the green callus tissue of finger millet at one week after subculture on MS medium with 0.2 mgl-1 2, 4-D and 0.1 mgl-1 kinetin. The protoplasts were release...
Organogenesis in Leaf Protoplast-Derived Calli of Mungbean
callus mungbean organogenesis protoplast root formation
2009/7/31
Protoplasts were successfully isolated from mungbean leaves (Vigna radiata L. Wilczek) using an enzyme solution containing 4% Meicelase P-1, 1% Macerozyme R-10, 1% potassium dextran sulphate and 0.5 ...
Effects of Iodoacetamide on the Fine Structure of Azuki Bean Protoplast and its Fusion Product
Azuki bean Fusion Iodoacetamide Protoplast Ultrastructure Viability
2009/7/27
Effects of iodoacetamide (IOA) on azuki bean protoplasts and their fusion products were examined with emphasis on thc fine structure. Protoplasts were isolated enzymatically from cell suspension cultu...
Production of Intergeneric Hybrid Calli from C3 and C4 Species of Amaranthaceae through Protoplast Fusion
Amaranthus tricolor L Celosia cristata L C4 species C3 species Electrofusion
2009/7/23
Intergeneric somatic hybrid calli were obtained through electrofusion between protoplasts of Celosia cristata L. (C3 species) cell suspension and Amaranthus tricolor L. (C4 species) cotyledon. The hig...
Changes in the Yield of Protoplast from Cryopreserved Rice Suspension Cells
Cell culture Cryopreservation Plasma membrane Protoplast Rice
2009/7/22
Changes in the Yield of Protoplast from Cryopreserved Rice Suspension Cells.
A Simple Procedure for Mesophyll Protoplast Culture and Plant Regeneration in Brassica oleracea L. and Brassica napus L
Brassica napus L Brassica oleracea L organogenesis protoplast culture regeneration
2014/3/13
An improved protocol for Brassica protoplast culture and plant regeneration was developed. Isolated
protoplasts from four-weeks-old in vitro shoot tip culture of Brassica oleracea var. botrytis cv...