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Short Communication: Effects of Isolation Stress on Mammary Tight Junctions in Lactating Dairy Cows
isolation stress endotoxin milk tight junction
2008/8/5
Eighteen cows had been selected for their responsiveness to psychological stress during the first lactation and were classified as having low (n = 10) or high (n = 8) cortisol concentrations in respon...
Spatial and Temporal Expression of Transferrin Gene In the Rat Mammary Gland
rat mammary transferrin in situ hybridization
2008/7/31
The distribution and concentration of transferrin mRNA in the rat mammary gland was analyzed using in situ hybridization, and the results were compared with those obtained for other milk protein mRNA....
Staphylococcus aureus Invasion of Bovine Mammary Epithelial Cells
invasion mastitis bovine Staphylococcus aureus
2008/7/31
Staphylococcus aureus is a frequent cause of mastitis in dairy cows. However, pathogenesis of the infection has not been completely defined. We report the invasion of two strains of S. aureus into a b...
Subcloning the MAC-T Bovine Mammary Epithelial Cell Line: Morphology, Growth Properties, and Cytogenetic Analysis of Clonal Cells
mammary epithelial cells MAC-T cells subcloning
2008/7/30
The objectives of the present study were 1) to determine the heterogeneity of the MAC-T cell line; 2) to examine whether homogeneous clones could be derived from MAC-T cells; and 3) to examine cell mo...
The Effect of Dietary Crude Protein as Protected Soybean Meal on Mammary Metabolism in the Lactating Dairy Cow
protein mammary gland metabolism lactation
2008/7/21
Metabolism in the mammary gland was related to changes in milk output in response to changes in dietary protein intake. Three diets of grass silage and concentrate were fed to four lactating dairy cow...
The Effect of Supplementary Protein on In Vivo Metabolism of the Mammary Gland in Lactating Dairy Cows
protein mammary gland metabolism lactation
2008/7/16
Four lactating cows equipped with rumen and duodenal cannulas were fed a diet of grass silage and concentrates containing either 12.4 or 17.2% CP (DM basis) in a change-over design. Additional protein...
The Response of Altering the Ratio of Dietary Protein to Energy on Growth, Feed Efficiency, and Mammary Developmentin Rapidly Growing Prepubertal Heifers
ADG ME Feed Efficiency
2008/7/4
This study was conducted to evaluate the effects of increasing the ratio of dietary protein to energy above National Research Council recommendations on average daily gain, feed efficiency, structural...
Two-Dimensional Electrophoretic Analysis of Proteins of Bovine Mammary Gland Secretions Collected During the Dry Period
mammary gland dry period milk proteins lactoferrin
2008/6/30
Proteins in mammary gland secretions, collected from Holstein cows during the dry period, were analyzed by preparative isoelectric focusing, followed by SDS-PAGE. Protein profiles changed throughout t...
Use of Bovine Primary Mammary Epithelial Cells for the Comparison of Adherence and Invasion Ability of Staphylococcus aureus Strains
Staphylococcus aureus adherence invasion epithelium
2008/6/27
Adherence and invasion of epithelial cells are thought to play a role in the pathogenesis of Staphylococcus aureus mastitis. A cell culture model with primary mammary epithelial cells originating from...
Alternative Splicing of Lactophorin mRNA from Lactating Mammary Gland of the Camel (Camelus dromedarius)
lactophorin proteose-peptone component 3 alternative mRNA splicing
2008/3/31
The objective of this study was to determine the corrected structure of lactophorin, a major whey protein in camel milk. The protein had 60.4% amino acid sequence identity to a proteose peptone compon...
The Effect of Mammary Gland Expression of Human Lysozyme on the Properties of Milk from Transgenic Mice
transgenic human lysozyme milk
2008/3/23
Transgenic mice were used as model systems to evaluate the impact of human lysozyme expression in the mammary gland. We previously generated two lines of transgenic mice that express human lysozyme mR...