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Background: Activation of the ileal brake through the delivery of nutrients into the distal small intestine to promote satiety and suppress food intake provides a new target for weight loss. Evidence ...
In silico prediction methods and tools were used to determine whether bioactive peptides are hidden in the amino acid sequences of carp (Cyprinus carpio) proteins and whether they can be released afte...
The rise in bacterial resistance and the declining approval rate of novel anti-infective drugs are a major threat to global public health. Antimicrobial peptides (AMPs), found in almost every multicel...
Iron is one of the most popular trace elements in body and plays very important role in metabolism or construction of body. Organic iron from various sources for curing iron-deficiency anemia was one ...
利用燕麦蛋白制备ACE抑制肽时先利用超声波对燕麦蛋白原料进行预处理研究。以ACE抑制活性和水解度为指标,考察了超声波功率、处理时间、超声波工作/间歇时间对燕麦蛋白预处理效果的影响。结果表明,超声波预处理的最佳工艺参数为:超声波功率500W,处理时间20min,超声波工作时间2 s、间歇时间2 s,该条件下酶解产物对ACE的半抑制浓度IC50值从0.533mg/mL降到0.299mg/mL...
采用酶法制备蛋清肽,色谱纯化ACE抑制活性组分并鉴定其一级结构。通过考察底物质量分数、加酶量、酶解温度和pH值对水解度的影响,结合多元线性回归设计和二次回归正交组合设计建立蛋清ACE抑制肽酶解工艺模型,并经液相色谱串联质谱鉴定其一级结构。结果表明:最佳酶解工艺为底物质量分数8%、pH值10.73、加酶量12.14%及酶解温度56.80℃,酶解物纯化后半抑制质量浓度为0.18mg/mL。液相色谱串...
This paper presents a modern in silico approach useful in the evaluation of proteins as a source of ACE inhibitors. All protein sequences analyzed were derived from the BIOPEP database. To determine t...
Bioinformatics methods have become one of the most important tools in peptide science. The number of available peptide databases is growing rapidly. The number of online programs able to process pepti...
Three peptides which inhibit angiotensin I-converting enzyme were isolated from maitake (Grifola frondosa) water extract digested by pepsin. Among them, Lys-Tyr-Thr-Phe-Ala-Val-Thr-Thr-Val-Lys-Thr-Trp...
Protease hydrolyses of an Okara protein yielded antioxidative activity against the peroxidation of linoleic acid in an aqueous system at pH 7.0. Four antioxidative peptides were isolated from the hydr...
Peptides having potent antioxidant activity were separated from the hydrolysate of wheat gluten by ion-exchange and gel filtration chromatography. Peptides obtained by SP Sephadex C-25 chromatography ...
Soybean protein isolate was hydrolyzed using two proteases (Protease M and Orientase 90N), and the inhibitory activity of angiotensin I-converting enzyme (ACE) and bitterness of the hydrolysates were ...
Several novel antioxidative peptides from the hydrolysate of water-soluble royal jelly protein (WSRJP) were isolated, identified, and characterized. WSRJP was extracted from royal jelly and hydrolyzed...
Five bitter-tasting peptides were isolated from charcoal-untreated sake, following a Sepabeads resin separation, an initial reverse-phase chromatography (RP-HPLC), a gel permeation-chromatography, and...
Controlled enzymatic modification proteins are currently being used as good sources of bioactive protein ingredients, and hydrolysates derived from bighead carp muscles may serve as antioxidants throu...

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