搜索结果: 1-13 共查到“动物细胞学 Embryos”相关记录13条 . 查询时间(0.124 秒)
Analysis of ENPP2 in the Uterine Endometrium of Pigs Carrying Somatic Cell Nuclear Transfer Cloned Embryos
Pig Pregnancy ENPP2 LPA SCNT
2016/5/12
Somatic cell nuclear transfer (SCNT) is a useful tool for animal cloning, but the efficiency of producing viable offspring by SCNT is very low. To improve this efficiency in the production of cloned p...
Phosphorylation Status of RNA Polymerase II Carboxyl-terminal Domain in Porcine Oocytes and Early Embryos
RNA Polymerase II Porcine Oocyte Embryos α-Amanitin
2016/5/10
Fertilization of the oocyte commences embryogenesis during which maternally inherited mRNAs are degraded and the embryonic genome is activated. Transcription of embryonic mRNA is initiated by embryoni...
Melatonin Attenuates Nitric Oxide Induced Oxidative Stress on Viability and Gene Expression in Bovine Oviduct Epithelial Cells, and Subsequently Increases Development of Bovine IVM/IVF Embryos
Melatonin Bovine Oviduct Epithelial Cell Bovine IVM/IVF Embryos Nitric Oxide Antioxidant
2016/5/6
The objective of the present study was to elucidate the fundamental mechanism of bovine oviduct epithelial cell (BOEC) co-culture on developmental capacity of bovine IVM/IVF embryos and to determine w...
Targeted Suppression of Connexin 43 in Ovine Preimplantation Embryos by RNA Interference Using Long Double-stranded RNA
Connexin 43 RNA Interference Ovine Embryo
2016/5/4
RNA interference (RNAi) is an acknowledged useful and effective tool to study gene function in various cells. Here, we suppressed the Connexin 43 (Cx 43) gene expression during in vitro development of...
Effects of Oviductal Fluid, Culture Media and Zona Pellucida Removal on the Development of Porcine Embryos by Nuclear Transfer
Oviductal Fluid Nuclear Transfer Culture Media Zona Pellucida Porcine
2016/4/29
The aim of this study was to compare the effects of oviductal fluid, porcine zygote medium (PZM)-3, PZM-4 and PZM-5, and modified PZM-5 culture media, and determine the effects of zona pellucida (ZP) ...
The Expression Patterns of Cdc25A, Cdc25B, Sox2 and Mnb in Central Nervous System in Early Chicken Embryos
Cdc25A Cdc25B Sox2 Mnb CNS In situ Hybridization Chicken Embryo
2016/4/29
The sense and antisense digoxigenin-labeled RNA probes of four genes, Cdc25A, Cdc25B, Sox2 and Mnb, were produced by using SP6 and T7 RNA polymerases, respectively, and in vitro transcription. Express...
Relationship between Developmental Ability and Cell Number of Day 2 Porcine Embryos Produced by Parthenogenesis or Somatic Cell Nuclear Transfer
Blastomeric Fragmentation Developmental Ability Embryo Quality Parthenogenesis Porcine Somatic Cell Nuclear Transfer
2016/4/29
In vitro produced porcine embryos have potential application in reproductive biotechnology. However, their development potential has been very low. This study evaluated the in vitro developmental abil...
In vitro Development of Interspecies Somatic Cell Nuclear Transfer Embryos Derived from Murine Embryonic Fibroblasts and Bovine Oocytes
Murine Bovine Interspecies Somatic Cell Nuclear Transfer b-Mercaptoethanol Xist
2016/4/28
Interspecies somatic cell nuclear transfer (iSCNT) is a useful method to preserve endangered species and to study the reprogramming event of a nuclear donor cell by the oocyte. Although several studie...
Effects of Cell Status of Bovine Oviduct Epithelial Cell (BOEC) on the Development of Bovine IVM/IVF Embryos and Gene Expression in the BOEC Used or Not Used for the Embryo Culture
Bovine Oviduct Epithelial Cell Bovine IVM/IVF Embryos Growth Factor Gene Apoptosis Gene Antioxidant Gene
2016/4/28
The objective of this study was to investigate the effects of cell status of BOEC on development of bovine IVM/IVF embryos and gene expression in BOEC before or after culturing of embryos. The develop...
Effects of Sperm Membrane Disruption and Electrical Activation of Oocytes on In vitro Development and Transgenesis of Porcine Embryos Produced by Intracytoplasmic Sperm Injection
Intracytoplasmic Sperm Injection GFP Transgenic Pigs
2016/4/27
The intracytoplasmic sperm injection (ICSI) procedure has recently been utilized to produce transgenic animals and may serve as an alternative to the conventional pronuclear microinjection in species ...
Effect of Oocyte Activation Regimens on Ploidy of Nuclear Transfer Embryos Reconstructed with Fetal Fibroblasts in Rabbit
Activation Nuclear Transfer Development Ploidy Rabbit
2016/4/25
Considerable attention has been focused on the cloning of mammalian embryos, as a consequence of poor development, in order to enhance the application of genetic engineering. Experiments were conducte...
The Influence of Microinjection of Foreign Gene into the Pronucleus of Fertilized Egg on the Preimplantation Development, Cell Number and Diameter of Rabbit Embryos
Rabbit Egg Blastocyst Microinjection Cell Number Embryo Diameter
2016/4/20
The aim of this in vitro study was to test the effect of microinjection (Mi) of foreign gene into the rabbit egg pronucleus and epidermal growth factor (EGF) addition on the blastocyst rate, the cell ...
Differential Influences in Sizes and Cell Cycle Stages of Donor Blastomeres on the Development of Cloned Rabbit Embryos
Nuclear Transfer Cell Cycle Stage Blastomere Cloned Embryo Rabbit
2016/4/12
Experiments were conducted to evaluate the effect of blastomere diameters and cell cycle stages on the subsequent development of nuclear transplant rabbit embryos (NT-embryos) using nuclei derived fro...