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Including transcription factor information in the superparamagnetic clustering of microarray data
Including transcription factor information superparamagnetic clustering
2011/1/5
In this work, we modify the superparamagnetic clustering algorithm (SPC) by adding an extra weight to the interaction formula that considers which genes are regulated by the same transcription factor....
摘要对寡核苷酸DNA Microarray用于HLADRB1基因分型的技术进行研究。常规的酚/氯仿法提取标准血样基因组DNA,在DRB1的exon2区域设计一对引物,经PCR扩增基因组相应区段并用Cy5-dCTP进行标记。设计寡核苷酸分型探针,将探针固定在APS-PDC法制作的DNA Microarray上,用标记的PCR产物与之杂交,扫描仪对杂交结果进行扫描,Imagene软件对杂交图象进行分析...