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Transcriptome profile of one-month-old lambs’ granulosa cells after superstimulation
Lamb Granulosa Cells Sheep RNA Seq Superstimulation
2017/3/7
Superstimulatory treatment of one-month-old lambs can achieve synchronous development of numerous growing follicles. However, these growing follicles cannot complete maturation and ovulation. Oocyte m...
Validation of Gene Silencing Using RNA Interference in Buffalo Granulosa Cells
RNAi Transfection CYP19 Gene 17beta- Estradiol Buffalo Transcript Abundance
2016/5/9
Silencing of a specific gene using RNAi (RNA interference) is a valuable tool for functional analysis of a target gene. However, information on RNAi for analysis of gene function in farm animals is re...
Influence of Nitric Oxide on Steroid Synthesis, Growth and Apoptosis of Buffalo (Bubalus bubalis) Granulosa Cells In vitro
Apoptosis Buffalo Granulosa Cell Nitric Oxide Steroidogenesis
2016/5/6
Objective of this study was to examine the effect of sodium nitroprusside (SNP), a nitric oxide (NO) donor on steroid synthesis, growth and apoptosis of buffalo granulosa cells (GCs) in vitro. Follicu...
Construction of Mammalian Cell Expression Vector for pAcGFP-bFLIP(L) Fusion Protein and Its Expression in Follicular Granulosa Cells
c-FLIP(L) pAcGFP-N l Recombinant Plasmid Follicular Granulosa Cell
2016/5/4
FLICE inhibitory protein (FLIP) is one of the important anti-apoptotic proteins in the Fas/FasL apoptotic path which has death effect domains, mimicking the pro-domain of procaspase-8. To reveal the i...
Effects of Daidzein on mRNA Expression of Bone Morphogenetic Protein Receptor Type I and II Genes in the Ovine Granulosa Cells
Daidzein Granulosa Cells Cell Proliferation BMP Receptor Genes
2016/5/4
Daidzein, a natural isoflavonoid phytoestrogen, structurally resembles estradiol (E2) and possesses estrogenic activity. This study was designed to test the hypothesis that daidzein may mimic the effe...
Effect of Arachidonic Acid on Production of Laminin and Connexin of Granulosa Cells from Chicken Pre-hierarchical Follicles
Granulosa Cell Arachidonic Acid Laminin Connexin 43 Chicken
2016/4/29
Arachidonic acid (AA) is a polyunsaturated fatty acid that is a normal constituent of membrane lipids in animal cells. In addition to its role as a precursor of prostaglandins, AA itself may play an i...
Changes in Number of Granulosa Cells, Follicular Fluid Levels and Diameter of Oocytes during Folliculogenesis in Pre-pubertal Gilts at Marketing Weight
Porcine Follicle Size Follicular Fluid Oocyte Granulosa Cell
2016/4/18
The follicles (1.8 to 7.8 mm in diameter) were recovered from the ovaries in marketed pigs and the number of granulosa cells, the diameter of oocytes obtained from different development stages of the ...
Albumin Fractions from Different Species Stimulate In Vitro Progesterone Production by Granulosa Cells in Buffalo
Serum Albumin Granulosa Cells In Vitro Steroidogenesis Buffalo
2016/4/12
The ovarian follicular fluid was found to contain steroidogenesis stimulatory protein similar to albumin from human and buffalo. Therefore, the albumins from various species, commercial and purified, ...
Intracellular Mechanisms of Growth Hormone Action on Apoptosis in Cultured Porcine Ovarian Granulosa Cells
Growth Hormone Protein Kinase G Tyrosine Kinase CDC Kinase Apoptosis Ovary
2016/4/11
The aims of this study were to detect spontaneously occurring apoptosis in cultured porcine ovarian cells, to examine the role of growth hormone (GH), tyrosine kinase (TK), protein kinase G (PKG) and ...