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Mechanisms of recruitment of the CTD phosphatase Rtr1 to RNA polymerase II
Rtr1 RNA polymerase II transcription
2015/5/25
The C-terminal domain (CTD) of the RNA polymerase II (RNAPII) subunit Rpb1 must exist in a hypophosphorylated state prior to forming a competent transcription initiation complex. However, during trans...
The role of the CTD phosphatase Rrt1 and post-translational modifications in regulation of RNA polymerase II
CTD phosphatase Rtr1 MuDPIT AP-MS
2015/5/25
RNA polymerase II (RNAPII) is regulated by multiple modifications to the C-terminal domain (CTD) of the largest subunit, Rpb1. This study has focused on the relationship between hyperphosphorylation o...
Rapid Origin Determination of the Northern Mauxia Shrimp (Acetes chinensis) Based on Allele Specific Polymerase Chain Reaction of Partial Mitochondrial 16S rRNA Gene
Allele-specific Polymerase Chain Reaction Origin Identification
2016/5/18
Acetes chinensis is an economically important shrimp that belongs to the Sergestidae family; following fermentation, A. chinensis′ economic value, however, is low in China, and much of the catch in Ch...
RNA-dependent RNA polymerase of turnip crinkle virus and the effect of coat protein on the replication of subviral RNAs
Biological sciences
2015/1/28
In this dissertation, I report the functions of the TCV coat protein in TCV viral and subviral RNA replication using in vivo and in vitro systems. In chapter 2, I present my results on construction of...
The stability of T7 RNA polymerase elongation complexes and promoter function analysis by in vitro selection
Forward translocation model polymerase complex stability DNA structure
2014/12/11
The forward translocation model (paused or stalled polymerase moves forward in the absence of transcription, dissociation of the complex happens when hybrid is too short to stabilize the complex) is t...
Functional tests of structural models for abortive cycling in T7 RNA polymerase
Pure sciences Biological sciences Abortive cycling RNA polymerase Transcription
2014/12/10
Transcription initiates when the enzyme binds to the promoter region and melts open an initial transcribing bubble that extends from position -4 to +4. The initiation phase continues until the enzyme ...
Mitochondrial DNA Polymerase IB: Functional Characterization of a Putative Drug Target for African Sleeping Sickness
kDNA kinetoplast DNA mitochondria RNA interference trypanosoma brucei trypanosome
2014/12/10
Trypanosoma brucei and related parasites are causative agents of severe diseases that affect global health and economy. T. brucei is responsible for sleeping sickness in humans (African trypanosomiasi...
Determination of Sperm Sex Ratio in Bovine Semen Using Multiplex Real-time Polymerase Chain Reaction
Sex Determination Sexed Semen Multiplex Real-time polymerase chain reaction
2016/5/17
Gender selection is important in livestock industries; for example, female calves are required in the dairy industry. Sex-sorted semen is commonly used for the production of calves of the desired gend...
Phosphorylation Status of RNA Polymerase II Carboxyl-terminal Domain in Porcine Oocytes and Early Embryos
RNA Polymerase II Porcine Oocyte Embryos α-Amanitin
2016/5/10
Fertilization of the oocyte commences embryogenesis during which maternally inherited mRNAs are degraded and the embryonic genome is activated. Transcription of embryonic mRNA is initiated by embryoni...
Sex Determination of Cattle Meat by Polymerase Chain Reaction Amplification of the DEAD Box Protein (DDX3X/DDX3Y) Gene
Cattle Meat Sex Determination Meat Quality DEAD Box Protein Gene
2016/5/10
Determination of sex origin of cattle meat by fast and reliable molecular methods is an important measure to ensure correct allocation of export refunds particularly in European countries and also fem...
Effect of Triticale Dried Distillers Grains with Solubles on Ruminal Bacterial Populations as Revealed by Real Time Polymerase Chain Reaction
Cattle Real Time PCR Rumen Microorganisms Triticale Dried Distillers Grains with Solubles
2016/5/9
Real time PCR was used in this study to determine the effect of triticale dried distillers grains with solubles (TDDGS) as a replacement for grain or barley silage in finishing diets on the presence o...
General-use polymerase chain reaction primers for amplification and direct sequencing of enolase, a single-copy nuclear gene, from different animal phyla
enolase Metazoa, scnDNA
2015/12/11
The dearth of general-use (i.e. quasi-‘universal’) primer
sets for the amplification of single-copy nuclear DNA
(scnDNA) loci, combined with the availability and ease of
use of mitochondrial (mtDNA...
Detection of the Paulownia Witches＇ Broom Mycoplasmalike Organism by Polymerase Chain Reaction
Paulownia witches’brom Mycoplasmalike organism polymerase chain reaction Pathogen diagnosis
2009/9/23
A polymerase chain reaction (PCR)method with high sensitivity and specificity has been developed
with a pair of oligonucleotide primers designed and synthesized according to the DNA sequence specific...
期刊信息
篇名
A modified procedure for fast purification of T7 RNA polymerase
语种
英文
撰写或编译
撰写
作者
Yong Li,En-duo Wang(通讯作者),Ying-lai Wang
第一作者单位
中科院上海生化所分子生物学国家重点实验室
刊物名称
Protein Expression and Purification,
...
Characterization of MHC DRB3.2 Alleles of Crossbred Cattle by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism
DRB3.2 Gene PCR-RFLP Crossbred Cattle
2016/4/20
The present investigation was undertaken to study the genetic polymorphism of the DRB3 exon 2 in 75 crossbred cattle by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP...